JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313412

Anti-FOXA2 (phospho T156) antibody [RP23040101]

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-Anti antibody [RP23040101] is a Rabbit Recombinant Multiclonal antibody that is used in Anti ICC/IF, Western Blot. Suitable for Human, Mouse samples.

FOXA2 is a transcription factor crucial for embryonic development and the regulation of tissue-specific gene expression. It plays a significant role in maintaining the function of differentiated tissues and is involved in processes such as glucose metabolism and liver function

View Alternative Names

HNF3B, TCF3B, FOXA2, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 (phospho T156) Antibody [RP23040101] (AB313412)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 (phospho T156) Antibody [RP23040101] (AB313412)

Immunofluorescent analysis of 70% confluent log phase HepG2 cells fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature labeling FOXA2 (phospho T156) with ab313412 at 1/400 dilution followed by Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing nuclear localization of FOXA2 (phospho T156). Panel e is no primary antibody control. Panel e shows competition with FOXA2 (phospho T156) peptide. The images were captured at 20X magnification.

Western blot - Anti-FOXA2 (phospho T156) Antibody [RP23040101] (AB313412)
  • WB

Supplier Data

Western blot - Anti-FOXA2 (phospho T156) Antibody [RP23040101] (AB313412)

All lanes:

Western blot - Anti-FOXA2 (phospho T156) Antibody [RP23040101] (ab313412) at 2 µg/mL

Lane 1:

HepG2 cell lysate at 30 µg

Lane 2:

Mouse liver tissue lysate at 30 µg

Lane 3:

phosphopeptide incubated (10 µg/mL) HepG2 cell lysate at 30 µg

Lane 4:

phosphopeptide incubated (10 µg/mL) Mouse liver tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG-HRP Secondary Antibody at 1/5000 dilution

Observed band size: 48 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040101

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC/IF

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "3-5 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.
Biological function summary

FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.

Pathways

FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.

Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
See full target information FOXA2 phospho T156

Additional targets

Foxa2 phospho T156
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Primary Antibodies

AB224637

Anti-SOX17 antibody [EPR20684]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SOX17 antibody [EPR20684] (AB224637)

  • 4
  • 6
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com