Rabbit Polyclonal FOXA3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXA3 aa 1-100.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FOXA3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P55318
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC1 promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.
Alternative names
HNF3G, TCF3G, FOXA3, Hepatocyte nuclear factor 3-gamma, HNF-3-gamma, HNF-3G, Fork head-related protein FKH H3, Forkhead box protein A3, Transcription factor 3G, TCF-3G
Recommended products
Rabbit Polyclonal FOXA3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXA3 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FOXA3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P55318
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXA3 also known as Forkhead box protein A3 is a transcription factor that belongs to the FOX family. It has a molecular mass of approximately 43 kDa. FOXA3 exhibits expression mainly in the liver pancreas and lung tissues where it regulates the transcription of genes involved in metabolic processes. It has a highly conserved DNA-binding domain known as the forkhead or winged helix domain which allows binding to specific DNA sequences to control gene expression.
- Biological function summary
FOXA3 plays an important role in the regulation of glucose and lipid homeostasis. It promotes the expression of genes involved in gluconeogenesis and lipogenesis processes. As a component of a transcriptional complex FOXA3 interacts with other transcription factors and co-activators to modulate target gene transcription. Its regulatory activities help maintain the balance of energy supply and storage in response to physiological needs.
- Pathways
FOXA3 participates in significant metabolic pathways that are essential for energy production and storage. One important pathway involves gluconeogenesis where FOXA3 partners with other transcription factors such as HNF4α to promote the expression of genes required for glucose synthesis. Additionally FOXA3 is involved in lipid metabolism pathways coordinating with proteins like PPARG to regulate lipogenic genes ensuring proper lipid storage and utilization in cells.
- Associated diseases and disorders
FOXA3 is linked to conditions like type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Aberrant regulation of FOXA3 can disturb glucose and lipid metabolism contributing to the development of these metabolic disorders. FOXA3 interacts with proteins such as PPARG and HNF4α which also have implications in these diseases by modulating pathways that affect insulin sensitivity and fat accumulation. Proper function of FOXA3 is essential for maintaining metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-FOXA3 antibody (ab222245)
4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FOXA3 (green) using ab222245 at 4 μg/ml in ICC/IF.
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