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AB222245

Anti-FOXA3 antibody

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(1 Publication)

Rabbit Polyclonal FOXA3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FOXA3 aa 1-100.

View Alternative Names

HNF3G, TCF3G, FOXA3, Hepatocyte nuclear factor 3-gamma, HNF-3-gamma, HNF-3G, Fork head-related protein FKH H3, Forkhead box protein A3, Transcription factor 3G, TCF-3G

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXA3 antibody (AB222245)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXA3 antibody (AB222245)

4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FOXA3 (green) using ab222245 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FOXA3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P55318

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXA3 also known as Forkhead box protein A3 is a transcription factor that belongs to the FOX family. It has a molecular mass of approximately 43 kDa. FOXA3 exhibits expression mainly in the liver pancreas and lung tissues where it regulates the transcription of genes involved in metabolic processes. It has a highly conserved DNA-binding domain known as the forkhead or winged helix domain which allows binding to specific DNA sequences to control gene expression.
Biological function summary

FOXA3 plays an important role in the regulation of glucose and lipid homeostasis. It promotes the expression of genes involved in gluconeogenesis and lipogenesis processes. As a component of a transcriptional complex FOXA3 interacts with other transcription factors and co-activators to modulate target gene transcription. Its regulatory activities help maintain the balance of energy supply and storage in response to physiological needs.

Pathways

FOXA3 participates in significant metabolic pathways that are essential for energy production and storage. One important pathway involves gluconeogenesis where FOXA3 partners with other transcription factors such as HNF4α to promote the expression of genes required for glucose synthesis. Additionally FOXA3 is involved in lipid metabolism pathways coordinating with proteins like PPARG to regulate lipogenic genes ensuring proper lipid storage and utilization in cells.

FOXA3 is linked to conditions like type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Aberrant regulation of FOXA3 can disturb glucose and lipid metabolism contributing to the development of these metabolic disorders. FOXA3 interacts with proteins such as PPARG and HNF4α which also have implications in these diseases by modulating pathways that affect insulin sensitivity and fat accumulation. Proper function of FOXA3 is essential for maintaining metabolic health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC1 promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.
See full target information FOXA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed38540147

2024

Circulating Exosomes from Septic Mice Activate NF-κB/MIR17HG Pathway in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Chun Wu,Cheng-Shyuan Rau,Yi-Chan Wu,Chia-Jung Wu,Chia-Wen Tsai,Lien-Hung Huang,Chia-Wei Lin,Tsu-Hsiang Lu,Ming-Yu Yang,Ching-Hua Hsieh
View all publications
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