Rabbit Polyclonal FOXB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOXB1 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FOXB1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99853
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transcription factor expressed by neural progenitor cells in specific regions of the embryonic neuroepithelium. Essential for the mammillary nuclei maintenance. Negatively regulates the proliferation of oligodendrocyte progenitors and promotes oligodendrocyte maturation. Also expressed in mammary glands, plays a role in lactation, controls development of mammary glands and the inferior colliculi of the midbrain in the central nervous system that regulates the milk-ejection reflex.
Alternative names
FKH5, FOXB1, Forkhead box protein B1, Transcription factor FKH-5
Recommended products
Rabbit Polyclonal FOXB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOXB1 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FOXB1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99853
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXB1 also known as Forkhead box protein B1 is a transcription factor. It typically weighs around 75 kDa. FOXB1 expression predominantly occurs in the brain where it regulates gene expression critical for development and function. This protein binds to DNA influencing the transcription of target genes which plays a role in development processes.
- Biological function summary
FOXB1 influences nervous system development and function acting as part of a complex that modulates transcriptional activity. Its role extends to maintaining cellular proliferation and differentiation. FOXB1's interaction with specific DNA elements shows its contribution to neurogenesis and axonal guidance which are essential for proper brain structure and function.
- Pathways
FOXB1 operates within the Hedgehog and Wnt signaling pathways which are essential for cell fate determination and growth in the brain. It works alongside proteins such as Gli in the Hedgehog signaling pathway to influence developmental processes. In the Wnt signaling pathway FOXB1 interacts with beta-catenin-related functions important for synaptic and neuronal activities in the brain.
- Associated diseases and disorders
FOXB1's involvement relates to neurodevelopmental disorders and glioblastoma. Altered FOXB1 expression or function can contribute to improper brain development leading to neurodevelopmental issues. In glioblastoma FOXB1 interacts with FoxG1 suggesting roles in tumor progression. Studying FOXB1 interactions offers insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-FOXB1 antibody - C-terminal (ab153947)
10% SDS PAGE
All lanes: Western blot - Anti-FOXB1 antibody - C-terminal (ab153947) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 20 kDa, 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FOXB1 antibody - C-terminal (ab153947)
Immunofluorescence analysis of paraformaldehyde-fixed HepG2, labeling FOXB1 with ab153947 at 1/200 dilution.
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