AB5060

Anti-FOXC2 antibody

Name: Anti-FOXC2 antibody (ab5060) | Abcam
Brand: Abcam Limited
SKU: AB5060
Price: 565 USD
Availability: InStock

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(33 Publications )

Goat Polyclonal FOXC2 antibody. Suitable for ChIP, WB and reacts with Mouse, African green monkey, Human, Transfected cell lysate, Transfected cell line samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Human FOXC2 aa 450 to C-terminus.

1 Images

Supplier Data

Western blot - Anti-FOXC2 antibody (AB5060)

Primary incubation : 1 hour at room temperature.

Negative Control : Human Pancreas lysate.

Lane 1:

Western blot - Anti-FOXC2 antibody (ab5060) at 2 µg/mL

Lane 2:

Western blot - Anti-FOXC2 antibody (ab5060) at 1 µg/mL

Lane 1:

nuclear HEK293 lysate in RIPA buffer at 35 µg

Lane 2:

human pancreas whole cell lysate (negative control) in RIPA buffer at 35 µg

Predicted band size: 53 kDa

Observed band size: 60-70 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey, Mouse

Applications

ChIP, WB

applications

Immunogen

Synthetic Peptide within Human FOXC2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99958

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-2 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "African green monkey": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell line": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-2 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell lysate": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXC2 also known as Forkhead Box C2 is a transcription factor weighing approximately 55 kDa. It is a part of the FOX family of transcription factors characterized by a distinct forkhead DNA-binding domain. FOXC2 expresses in various tissues including adipose tissue blood vessels and mesenchymal cells. Its role is to regulate the expression of genes involved in development and differentiation.

Biological function summary

This transcription factor plays a significant role in the development of the cardiovascular and lymphatic systems. It functions as part of a complex network working with other transcription factors to control gene expression that governs vascular remodeling and lymphangiogenesis. Through its activity FOXC2 influences cell fate decisions impacting tissue formation and function during embryogenesis and adult life.

Pathways

FOXC2 participates notably in the TGF-beta signaling pathway. It interacts with SMAD family proteins to influence cellular responses to transforming growth factor-beta signals which are important for cellular proliferation and differentiation. FOXC2 also interfaces with other signaling pathways working in tandem with proteins like Notch and VEGF to modulate angiogenesis and vascular homeostasis.

FOXC2 mutations and dysregulation associate with lymphedema-distichiasis syndrome and various types of cancer. It frequently interacts with other proteins such as VEGFR-3 in these conditions. Mutations affecting FOXC2 can lead to abnormal lymphatic development contributing to fluid retention and limb swelling in lymphedema. In cancer FOXC2 acts as a regulator of cell invasion and metastasis indicating its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator.

See full target information FOXC2

Alternative Names

FKHL14, MFH1, FOXC2, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14, MFH-1 protein

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40464693

2025

A role for pH dynamics regulating transcription factor DNA-binding selectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle P Kisor,Diego Garrido Ruiz,Matthew P Jacobson,Diane L Barber

Nucleic acids research 52:6269-6284 PubMed38634789

2024

Proximal telomeric decompaction due to telomere shortening drives FOXC1-dependent myocardial senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Weiyao Xiong,Wu Zuo,Yuanyuan Shi,Teng Wang,Lingling Chang,Yueheng Wu,Heng Ma,Qian Bian,Alex C Y Chang

Cells 12: PubMed37887278

2023

Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Yang Yang,Yan Zhao,Xiuju Wu,Li Zhang,Xinjiang Cai,Jaden Ji,Kristina I Boström,Yucheng Yao

International journal of biological sciences 19:4206-4222 PubMed37705741

2023

FOXC2-AS1/FOXC2 axis mediates matrix stiffness-induced trans-differentiation of hepatic stellate cells into fibrosis-promoting myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Liankang Sun,Yue Li,Hao Wang,Xuelian Xiao,Xuenan Luo,Ruida Yang,Jinyan Li,Yifei Ma,Qingguang Liu,Kangsheng Tu,Yu Shi

Cells 12: PubMed37508475

2023

Cell Transitions Contribute to Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Xiuju Wu,Yan Zhao,Yang Yang,Li Zhang,Xinjiang Cai,Jocelyn A Ma,Jaden Ji,Karen Lyons,Kristina I Boström,Yucheng Yao

Journal of experimental & clinical cancer research : CR 41:129 PubMed35392966

2022

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feikai Lin,Xiaoduan Li,Xinjing Wang,Huizhen Sun,Ziliang Wang,Xipeng Wang

Frontiers in oncology 11:634579 PubMed33869020

2021

The FENDRR/FOXC2 Axis Contributes to Multidrug Resistance in Gastric Cancer and Correlates With Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Zhe Zhang,Yanan Han,Ahui Fan,Haiming Liu,Xiangyuan Zhang,Yanhong Liu,Rugang Zhang,Wanning Liu,Yuanyuan Lu,Daiming Fan,Xiaodi Zhao,Yongzhan Nie

Translational cancer research 9:5268-5280 PubMed35117893

2020

Expression of combined interference of slug and FoxC2 in endometrial carcinoma and its clinicopathological relationship.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhu,Mingyang Tang,Ligao Wu

International journal of molecular sciences 21: PubMed32698337

2020

Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Tavian,Sara Missaglia,Sandro Michelini,Paolo Enrico Maltese,Elena Manara,Alvaro Mordente,Matteo Bertelli

eLife 9: PubMed32510325

2020

Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter R Norden,Amélie Sabine,Ying Wang,Cansaran Saygili Demir,Ting Liu,Tatiana V Petrova,Tsutomu Kume

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FOXC2 antibody

Western blot - Anti-FOXC2 antibody (AB5060)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (33)

Product promise