Rabbit Recombinant Monoclonal FOXC2 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXC2 aa 350-400.
Images
![Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--western-blot-img420390.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)")
![Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--western-blot-img420391.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)")
![Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunoprecipitation-img420392.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)")
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img428137.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)")
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img420394.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human FOXC2 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99958
Reactivity data
Select an application| ICC | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Alternative names
FKHL14, MFH1, FOXC2, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14, MFH-1 protein
Recommended products
Rabbit Recombinant Monoclonal FOXC2 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXC2 aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human FOXC2 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99958
- Clone number
- BLR184J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXC2 also known as Forkhead Box C2 is a transcription factor weighing approximately 55 kDa. It is a part of the FOX family of transcription factors characterized by a distinct forkhead DNA-binding domain. FOXC2 expresses in various tissues including adipose tissue blood vessels and mesenchymal cells. Its role is to regulate the expression of genes involved in development and differentiation.
- Biological function summary
This transcription factor plays a significant role in the development of the cardiovascular and lymphatic systems. It functions as part of a complex network working with other transcription factors to control gene expression that governs vascular remodeling and lymphangiogenesis. Through its activity FOXC2 influences cell fate decisions impacting tissue formation and function during embryogenesis and adult life.
- Pathways
FOXC2 participates notably in the TGF-beta signaling pathway. It interacts with SMAD family proteins to influence cellular responses to transforming growth factor-beta signals which are important for cellular proliferation and differentiation. FOXC2 also interfaces with other signaling pathways working in tandem with proteins like Notch and VEGF to modulate angiogenesis and vascular homeostasis.
- Associated diseases and disorders
FOXC2 mutations and dysregulation associate with lymphedema-distichiasis syndrome and various types of cancer. It frequently interacts with other proteins such as VEGFR-3 in these conditions. Mutations affecting FOXC2 can lead to abnormal lymphatic development contributing to fluid retention and limb swelling in lymphedema. In cancer FOXC2 acts as a regulator of cell invasion and metastasis indicating its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--western-blot-img420390.jpg "Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] (A700-184 lot 1) used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051) at 1/1000 dilution
Lane 1: NIH 3T3 Whole cell lysate at 50 µg
Lane 2: CT 26 Whole cell lysate at 50 µg
Lane 3: CH 27 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG
![Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--western-blot-img420391.jpg "Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Samples: Whole cell lysate (50 µg) from HeLa, HeLa NE (25 µg), OVCAR-3, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F]
All lanes: Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 50 µg
Lane 2: HeLa NE Whole cell lysate at 50 µg
Lane 3: OVCAR-3 Whole cell lysate at 50 µg
Lane 4: MCF-7Whole cell lysate at 50 µg
Lane 5: GaMG Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunoprecipitation-img420392.jpg "Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Samples: HeLa nuclear extract (1.0 mg per IP reaction; 5% of IP loaded). Antibodies: Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] used for IP at 20 µl/mg lysate. FOXC2 was also immunoprecipitated by a second antibody against a different epitope of FOXC2 (BL-1796A-1A7). For blotting immunoprecipitated FOXC2, A700-184 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img428137.jpg "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Detection of human FOXC2 in HeLa cells by ICC. Antibody: Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img420394.jpg "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Detection of human FOXC2 in Ovcar-3 cells by ICC . Antibody: Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] (A700-184 lot 1). Secondary: HRPconjugated goat anti-rabbit IgG . Substrate: DAB
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img420393.jpg "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)
Detection of mouse FOXC2 in 3T3 cells by ICC.Antibody: Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB
![Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051), expandable thumbnail](https://content.abcam.com/products/images/foxc2-antibody-blr184j-bsa-free-ab314051--immunocytochemistry-img420393.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051)")
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