AB314051

Anti-FOXC2 antibody [BLR184J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal FOXC2 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXC2 aa 350-400.

View Alternative Names

FKHL14, MFH1, FOXC2, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14, MFH-1 protein

6 Images

Supplier Data

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Detection of human FOXC2 in HeLa cells by ICC. Antibody : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J]. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB

Supplier Data

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Detection of human FOXC2 in Ovcar-3 cells by ICC . Antibody : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] (A700-184 lot 1). Secondary : HRPconjugated goat anti-rabbit IgG . Substrate : DAB

Supplier Data

Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Samples : HeLa nuclear extract (1.0 mg per IP reaction; 5% of IP loaded). Antibodies : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] used for IP at 20 µl/mg lysate. FOXC2 was also immunoprecipitated by a second antibody against a different epitope of FOXC2 (BL-1796A-1A7). For blotting immunoprecipitated FOXC2, A700-184 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

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Supplier Data

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Detection of mouse FOXC2 in 3T3 cells by ICC.Antibody : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J]. Secondary : HRP-conjugated goat anti-rabbit IgG Substrate : DAB

Supplier Data

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Samples : Whole cell lysate (50 µg) from HeLa, HeLa NE (25 µg), OVCAR-3, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F]

All lanes:

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HeLa NE Whole cell lysate at 50 µg

Lane 3:

OVCAR-3 Whole cell lysate at 50 µg

Lane 4:

MCF-7Whole cell lysate at 50 µg

Lane 5:

GaMG Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Supplier Data

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-FOXC2 recombinant monoclonal antibody [BLR184J] (A700-184 lot 1) used at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (ab314051) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT 26 Whole cell lysate at 50 µg

Lane 3:

CH 27 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR184J

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, WB, ICC

applications

Immunogen

Synthetic Peptide within Human FOXC2 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q99958

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXC2 also known as Forkhead Box C2 is a transcription factor weighing approximately 55 kDa. It is a part of the FOX family of transcription factors characterized by a distinct forkhead DNA-binding domain. FOXC2 expresses in various tissues including adipose tissue blood vessels and mesenchymal cells. Its role is to regulate the expression of genes involved in development and differentiation.

Biological function summary

This transcription factor plays a significant role in the development of the cardiovascular and lymphatic systems. It functions as part of a complex network working with other transcription factors to control gene expression that governs vascular remodeling and lymphangiogenesis. Through its activity FOXC2 influences cell fate decisions impacting tissue formation and function during embryogenesis and adult life.

Pathways

FOXC2 participates notably in the TGF-beta signaling pathway. It interacts with SMAD family proteins to influence cellular responses to transforming growth factor-beta signals which are important for cellular proliferation and differentiation. FOXC2 also interfaces with other signaling pathways working in tandem with proteins like Notch and VEGF to modulate angiogenesis and vascular homeostasis.

FOXC2 mutations and dysregulation associate with lymphedema-distichiasis syndrome and various types of cancer. It frequently interacts with other proteins such as VEGFR-3 in these conditions. Mutations affecting FOXC2 can lead to abnormal lymphatic development contributing to fluid retention and limb swelling in lymphedema. In cancer FOXC2 acts as a regulator of cell invasion and metastasis indicating its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator.

See full target information FOXC2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXC2 antibody [BLR184J] - BSA free

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Immunoprecipitation - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Immunocytochemistry - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Western blot - Anti-FOXC2 antibody [BLR184J] - BSA free (AB314051)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise