Anti-FOXC2 antibody [RP23040265] - ChIP Grade

• ChIP

Supplier Data

ChIP - Anti-FOXC2 antibody [RP23040265] - ChIP Grade (AB308055)

Chromatin Immunoprecipitation (ChIP) was performed using 4 μg ab308055 on sheared chromatin from 2 million HeLa cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of MET, ANGPT2, ITGB3 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

• WB

Supplier Data

Western blot - Anti-FOXC2 antibody [RP23040265] - ChIP Grade (AB308055)

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of SW480 (Lane 1), Caco-2 (Lane 2) and HT-29 (Lane 3). The blot was probed with ab308055 at 1 : 500 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1 : 4000 dilution. A 60 kDa band corresponding to FOXC2 was observed across the cell lines tested.

All lanes:

Western blot - Anti-FOXC2 antibody [RP23040265] - ChIP Grade (ab308055) at 1/500 dilution

Lane 1:

SW480 whole cell extract at 30 µg

Lane 2:

Caco-2 whole cell extract at 30 µg

Lane 3:

HT-29 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 60 kDa

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• WB

Supplier Data

Western blot - Anti-FOXC2 antibody [RP23040265] - ChIP Grade (AB308055)

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of DU 145 (Lane 1) and LNCaP (Lane 2). The blot was probed with ab308055 at 1 : 500 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1 : 4000 dilution. A ~60 kDa band corresponding to FOXC2 was observed across the cell lines tested.

Lanes 1 - 2:

Western blot - Anti-FOXC2 antibody [RP23040265] - ChIP Grade (ab308055) at 1/500 dilution

Lane 1:

DU-145 whole cell extract at 30 µg

Lane 2:

LNCap whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 60 kDa

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