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AB129324

Anti-FOXD1/BF-2 antibody

2

(1 Review)

|

(8 Publications)

Goat Polyclonal FOXD1/BF-2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human FOXD1 aa 450 to C-terminus.

View Alternative Names

FKHL8, FREAC4, FOXD1, Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4, FREAC-4

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (AB129324)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (AB129324)

ab129324, at 2.5 μg/ml, staining FOXD1/BF-2 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FOXD1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16676

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Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXD1 also known as BF-2 acts as a transcription factor with a molecular weight of approximately 51 kDa. It binds to DNA to regulate gene expression influencing the transcription of down-stream genes. FOXD1 expression is most prominent in the kidney as well as in various regions of the brain and retina. The protein's action is important for development and differentiation processes in these tissues.
Biological function summary

The activity of FOXD1/BF-2 is central to organogenesis especially in the kidneys where it regulates nephron progenitor cells. It forms part of a gene regulatory complex that modulates the expression of other transcription factors necessary for organ development. Beyond organogenesis FOXD1 influences the function of the neural crest-derived tissues and contributes to patterning during embryonic development.

Pathways

The FOXD1/BF-2 protein integrates into the renin-angiotensin system impacting fluid homeostasis and blood pressure regulation. Additionally it participates in the Wnt signaling pathway an important pathway in cell fate determination. FOXD1 interacts with other proteins such as PRRX1 and GATA3 therefore solidifying its role in these signaling cascades.

FOXD1's dysfunction has links to renal dysplasia and hypertension. In renal dysplasia the improper expression of FOXD1 alters kidney development which affects renal function. Additionally FOXD1 alteration can relate to hypertensive disorders due to its role in the renin-angiotensin system. The interaction with PRRX1 highlights its involvement in these conditions causing ripple effects across the associated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in regulation of gene expression in a variety of processes, including formation of positional identity in the developing retina, regionalization of the optic chiasm, morphogenesis of the kidney, and neuralization of ectodermal cells (By similarity). Involved in transcriptional activation of PGF and C3 genes (PubMed : 27805902).
See full target information FOXD1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40183774

2025

Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation.

Applications

Unspecified application

Species

Unspecified reactive species

Mehmet Mahsum Kaplan,Erika Hudacova,Miroslav Matejcek,Haneen Tuaima,Jan Křivánek,Ondrej Machon

CytoJournal 21:71 PubMed39917004

2025

Identification of transcription factors associated with the disease-free survival of triple-negative breast cancer through weighted gene co-expression network analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Huipo Wang,Ran Hao,Wei Liu,Yi Zhang,Shen Ma,Yiwei Lu,Jie Hu,Yixin Qi

PeerJ 12:e18406 PubMed39494294

2024

Forkhead box D subfamily genes in colorectal cancer: potential biomarkers and therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Haiyan Qiao,Ruiqi Zhong,Lei Sun,Bingbing Shang

BioMed research international 2022:7798654 PubMed35607308

2022

The Expression and Survival Significance of FOXD1 in Lung Squamous Cell Carcinoma: A Meta-Analysis, Immunohistochemistry Validation, and Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xie,Yunhui Li,Bin Liang

BMC cancer 21:1205 PubMed34772357

2021

Aberrant overexpression of transcription factor Forkhead box D1 predicts poor prognosis and promotes cancer progression in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Li,Tingyuan Yan,Xiang Wu,Xueping Ke,Xin Li,Yumin Zhu,Jianrong Yang,Zhongwu Li

Bioscience reports 41: PubMed34269372

2021

FOXD1 expression in head and neck squamous carcinoma: a study based on TCGA, GEO and meta-analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Huang,Bin Liang,Tianjiao Wang

Cell stem cell 25:373-387.e9 PubMed31303547

2019

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hui Low,Pin Li,Elaine Guo Yan Chew,Bingrui Zhou,Keiichiro Suzuki,Tian Zhang,Michelle Mulan Lian,Meng Liu,Emi Aizawa,Concepcion Rodriguez Esteban,Kylie Su Mei Yong,Qingfeng Chen,Josep M Campistol,Mingliang Fang,Chiea Chuen Khor,Jia Nee Foo,Juan Carlos Izpisua Belmonte,Yun Xia

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:7037-7048 PubMed30870006

2019

Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Lei,Hui-Qing Li,Zhi-Huai Mo,Ke-Jia Liu,Ling-Juan Zhu,Chun-Yi Li,Wen-Li Chen,Lei Zhang
View all publications
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Product promise

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