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AB237030

Anti-FOXE3 antibody

1

(1 Review)

|

(1 Publication)

Rabbit Polyclonal FOXE3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FOXE3 aa 1-100.

View Alternative Names

FKHL12, FREAC8, FOXE3, Forkhead box protein E3, Forkhead-related protein FKHL12, Forkhead-related transcription factor 8, FREAC-8

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXE3 antibody (AB237030)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXE3 antibody (AB237030)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FOXE3 using ab237030 at 1/166 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FOXE3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q13461

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXE3 also known as forkhead box E3 is a transcription factor weighing approximately 33 kDa. It exhibits expression predominantly in the eye lens particularly during development. As a member of the forkhead family of transcription factors FOXE3 plays key roles in the regulation of gene expression. Mammalian species show high conservation of FOXE3 gene sequences reflecting its importance across different organisms.
Biological function summary

Interaction with other proteins is important for FOXE3's function particularly in the differentiation of lens fiber cells and lens development. This transcription factor ensures proper lens morphogenesis maintaining transparency and preventing cataract formation. Though its primary activity lies in the eye FOXE3 can influence other tissues by regulating gene expression pertinent to epithelial cell development. Current data suggests FOXE3 does not form large multi-protein complexes but interacts with specific gene promoters to exert its function.

Pathways

FOXE3 activity integrates into the lens development and morphogenesis pathways. It regulates genes such as CRYAA and MIP important for lens transparency and cell differentiation. FOXE3 works closely with PAX6 and SOX2 proteins that are also pivotal in the eye development pathway. These interactions highlight its integral role in eye development processes and maintenance of tissue homeostasis within the ocular microenvironment.

FOXE3 mutations or dysregulation associate with congenital cataracts and anterior segment dysgenesis. Anomalies in FOXE3 expression can cause severe developmental disorders in the eye leading to visual impairment or blindness. The PAX6 gene can also contribute to similar disorders establishing a genetic linkage between the two in their disease manifestations. Researchers continue to explore FOXE3's involvement in these pathologies to develop potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that controls lens epithelial cell growth through regulation of proliferation, apoptosis and cell cycle (PubMed : 22527307, PubMed : 25504734). During lens development, controls the ratio of the lens fiber cells to the cells of the anterior lens epithelium by regulating the rate of proliferation and differentiation (By similarity). Controls lens vesicle closure and subsequent separation of the lens vesicle from ectoderm (By similarity). Controls the expression of DNAJB1 in a pathway that is crucial for the development of the anterior segment of the eye (PubMed : 27218149).
See full target information FOXE3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 30:e13562 PubMed38279604

2024

Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Aixue,Wei Feng,Zhang Huanhuan,M Xixing,L Yanling
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