Rabbit Polyclonal FOXE3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXE3 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FOXE3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q13461
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
Transcription factor that controls lens epithelial cell growth through regulation of proliferation, apoptosis and cell cycle (PubMed:22527307, PubMed:25504734). During lens development, controls the ratio of the lens fiber cells to the cells of the anterior lens epithelium by regulating the rate of proliferation and differentiation (By similarity). Controls lens vesicle closure and subsequent separation of the lens vesicle from ectoderm (By similarity). Controls the expression of DNAJB1 in a pathway that is crucial for the development of the anterior segment of the eye (PubMed:27218149).
Alternative names
FKHL12, FREAC8, FOXE3, Forkhead box protein E3, Forkhead-related protein FKHL12, Forkhead-related transcription factor 8, FREAC-8
Recommended products
Rabbit Polyclonal FOXE3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXE3 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FOXE3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q13461
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXE3 also known as forkhead box E3 is a transcription factor weighing approximately 33 kDa. It exhibits expression predominantly in the eye lens particularly during development. As a member of the forkhead family of transcription factors FOXE3 plays key roles in the regulation of gene expression. Mammalian species show high conservation of FOXE3 gene sequences reflecting its importance across different organisms.
- Biological function summary
Interaction with other proteins is important for FOXE3's function particularly in the differentiation of lens fiber cells and lens development. This transcription factor ensures proper lens morphogenesis maintaining transparency and preventing cataract formation. Though its primary activity lies in the eye FOXE3 can influence other tissues by regulating gene expression pertinent to epithelial cell development. Current data suggests FOXE3 does not form large multi-protein complexes but interacts with specific gene promoters to exert its function.
- Pathways
FOXE3 activity integrates into the lens development and morphogenesis pathways. It regulates genes such as CRYAA and MIP important for lens transparency and cell differentiation. FOXE3 works closely with PAX6 and SOX2 proteins that are also pivotal in the eye development pathway. These interactions highlight its integral role in eye development processes and maintenance of tissue homeostasis within the ocular microenvironment.
- Associated diseases and disorders
FOXE3 mutations or dysregulation associate with congenital cataracts and anterior segment dysgenesis. Anomalies in FOXE3 expression can cause severe developmental disorders in the eye leading to visual impairment or blindness. The PAX6 gene can also contribute to similar disorders establishing a genetic linkage between the two in their disease manifestations. Researchers continue to explore FOXE3's involvement in these pathologies to develop potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-FOXE3 antibody (ab237030)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FOXE3 using ab237030 at 1/166 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
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