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Rabbit Recombinant Monoclonal FOXF1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-FOXF1 antibody [EPR27368-79] (AB308633), expandable thumbnail
  • Immunoprecipitation - Anti-FOXF1 antibody [EPR27368-79] (AB308633), expandable thumbnail
  • Western blot - Anti-FOXF1 antibody [EPR27368-79] (AB308633), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (AB308633), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (AB308633), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBIHC-PIP
Human
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Probable transcription activator for a number of lung-specific genes.

Alternative names

FKHL5, FREAC1, FOXF1, Forkhead box protein F1, Forkhead-related activator 1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FREAC-1

Recommended products

Rabbit Recombinant Monoclonal FOXF1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27368-79
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXF1 also known as Forkhead Box F1 is a transcription factor with a molecular mass of approximately 47 kDa. This protein belongs to the forkhead family which is characterized by a distinct forkhead DNA-binding domain. FOXF1 is expressed mainly in the mesenchyme of developing organs such as the lung and gut where it plays an essential role in organogenesis and development.

Biological function summary

FOXF1 regulates gene expression involved in cellular differentiation and embryonic development. It acts as a transcriptional activator binding specific DNA sequences to initiate transcription. FOXF1 does not function in isolation; it forms part of transcriptional complexes by interacting with other regulatory proteins enhancing its role as a transcription factor in specific tissue types. Through these interactions FOXF1 influences the development of organs and tissues.

Pathways

FOXF1 actively participates in the Wnt and Hedgehog signaling pathways both critical for embryonic development and cellular proliferation. In the Wnt pathway FOXF1 interacts with β-catenin a central protein in the pathway to regulate target gene expression that controls cell differentiation and proliferation. In the Hedgehog pathway FOXF1 modulates gene expression alongside transcription factors such as GLI proteins controlling various developmental processes.

Associated diseases and disorders

FOXF1 mutations or dysregulation have strong associations with congenital diaphragmatic hernia and alveolar capillary dysplasia both of which are severe developmental conditions. FOXF1 dysregulation disrupts normal lung and diaphragm development linking it to these disorders. Additionally the Hedgehog pathway where FOXF1 plays a role involves GLI proteins tying FOXF1 indirectly to certain developmental syndromes when mutations are present.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Low expression: A375 (PMID: 29162563).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29162563, 11313147).

    In lane 1, the lysate was stored at -80C°C prior to Western Blotting.  The bands beneath the target band (40 kDa) are likey to be degradation products. In lane 3, the lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

    Observed band size: 40 kDa

    Exposure time: 37s

  • Immunoprecipitation - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Immunoprecipitation - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    FOXF1 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate with ab308633 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308633 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse lung tissue lysate

    Lane 2: abAB308633 IP in Mouse lung tissue lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308633 in mouse lung tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-FOXF1 antibody [EPR27368-79] (ab308633) at 1/30 dilution

    All lanes: Mouse lung tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 40 kDa

    Exposure time: 180s

  • Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    All lanes: Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633) at 1/1000 dilution

    All lanes: Rat lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

    Observed band size: 40 kDa

    Exposure time: 103s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling FOXF1 with ab308633 at 1/1000 (0.52 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: no staining on rat cardiac muscle. The section was incubated with ab308633 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling FOXF1 with ab308633 at 1/1000 (0.52 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: no staining on mouse cardiac muscle (PMID: 11313147). The section was incubated with ab308633 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling FOXF1 with ab308633 at 1/1000 (0.52 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat lung. The section was incubated with ab308633 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling FOXF1 with ab308633 at 1/1000 (0.52 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse lung (PMID: 11313147). The section was incubated with ab308633 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633), expandable thumbnail

    Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Low expression: colon (PMID: 11313147, 7957066), heart (PMID: 11313147, 7957066).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29162563, 11313147).

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-FOXF1 antibody [EPR27368-79] (ab308633) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: Mouse colon tissue lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

    Observed band size: 40 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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