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AB168383

Anti-FOXF1 antibody [EPR7971]

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(7 Publications)

Anti-FOXF1 antibody [EPR7971] (ab168383) is a rabbit monoclonal antibody detecting FOXF1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

FKHL5, FREAC1, FOXF1, Forkhead box protein F1, Forkhead-related activator 1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FREAC-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (AB168383)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (AB168383)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1 : 250 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] (AB168383)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] (AB168383)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1/70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)
  • WB

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

This antibody detects high background

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Human lung lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)
  • WB

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/1000 dilution

Lane 1:

Frozen HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Fresh HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-FOXF1 antibody [EPR7971]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-FOXF1 antibody [EPR7971]

  • Carrier free

    Anti-FOXF1 antibody [EPR7971] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-FOXF1 antibody [EPR7971]

  • 660 APC

    APC Anti-FOXF1 antibody [EPR7971]

  • 578 PE

    PE Anti-FOXF1 antibody [EPR7971]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7971

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-FOXF1 antibody [EPR7971] (ab168383) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of FOXF1?
Anti-FOXF1 [EPR7971] (ab168383) specifically detects a band for FOXF1 (UniProt: Q12946) at a molecular weight of 40kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR7971] also available for your convenience: ab168383, Alexa Fluor® 488 - ab214875, Alexa Fluor® 647 - ab215940, Carrier free - ab240153, APC - ab319331, PE - ab319468, Alexa Fluor® 555 - ab319965

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXF1 also known as Forkhead Box F1 is a transcription factor with a molecular mass of approximately 47 kDa. This protein belongs to the forkhead family which is characterized by a distinct forkhead DNA-binding domain. FOXF1 is expressed mainly in the mesenchyme of developing organs such as the lung and gut where it plays an essential role in organogenesis and development.
Biological function summary

FOXF1 regulates gene expression involved in cellular differentiation and embryonic development. It acts as a transcriptional activator binding specific DNA sequences to initiate transcription. FOXF1 does not function in isolation; it forms part of transcriptional complexes by interacting with other regulatory proteins enhancing its role as a transcription factor in specific tissue types. Through these interactions FOXF1 influences the development of organs and tissues.

Pathways

FOXF1 actively participates in the Wnt and Hedgehog signaling pathways both critical for embryonic development and cellular proliferation. In the Wnt pathway FOXF1 interacts with Β-catenin a central protein in the pathway to regulate target gene expression that controls cell differentiation and proliferation. In the Hedgehog pathway FOXF1 modulates gene expression alongside transcription factors such as GLI proteins controlling various developmental processes.

FOXF1 mutations or dysregulation have strong associations with congenital diaphragmatic hernia and alveolar capillary dysplasia both of which are severe developmental conditions. FOXF1 dysregulation disrupts normal lung and diaphragm development linking it to these disorders. Additionally the Hedgehog pathway where FOXF1 plays a role involves GLI proteins tying FOXF1 indirectly to certain developmental syndromes when mutations are present.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable transcription activator for a number of lung-specific genes.
See full target information FOXF1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37539708

2023

Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yining Hao,Wei He,Haofei Wang,Wenbin Rui,Fukang Sun,Yu Zhu,Danfeng Xu,Chenghe Wang

Experimental and therapeutic medicine 25:103 PubMed36798677

2023

FOXF1 attenuates TGF‑β1‑induced bronchial epithelial cell injury by inhibiting CDH11‑mediated Wnt/β‑catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Chen,Xing Liao,Ling Lin,Ling Wu,Qiuyu Tang

Stem cells international 2021:9964159 PubMed34257670

2021

Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Han,Yanhui Zhang,Yuxia Xiao,Bohong Shi,Hong Wu,Desheng Liu

Methods in molecular biology (Clifton, N.J.) 2214:41-58 PubMed32944902

2020

Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie M Morgani,Anna-Katerina Hadjantonakis

Cell reports 25:3215-3228.e9 PubMed30540952

2018

A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Su Su,Anyonya R Guntur,Daniel C Nguyen,Shameem S Fakory,Chad C Doucette,Cassandra Leech,Humphrey Lotana,Matthew Kelley,Jaspreet Kohli,Julieta Martino,Sunder Sims-Lucas,Lucy Liaw,Calvin Vary,Clifford J Rosen,Aaron C Brown

Genome biology 19:118 PubMed30119690

2018

Functional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Han,Li Li,Alejandro Piñeiro Ugalde,Arieh Tal,Zohar Manber,Eric Pinto Barbera,Veronica Della Chiara,Ran Elkon,Reuven Agami

Cancer discovery 8:234-251 PubMed29162563

2017

FOXF1 Defines the Core-Regulatory Circuitry in Gastrointestinal Stromal Tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Leili Ran,Yuedan Chen,Jessica Sher,Elissa W P Wong,Devan Murphy,Jenny Q Zhang,Dan Li,Kemal Deniz,Inna Sirota,Zhen Cao,Shangqian Wang,Youxin Guan,Shipra Shukla,Katie Yang Li,Alan Chramiec,Yuanyuan Xie,Deyou Zheng,Richard P Koche,Cristina R Antonescu,Yu Chen,Ping Chi
View all publications
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