Anti-FOXG1 antibody is a rabbit polyclonal antibody that is used in forkhead box protein G1 (FOXG1) western blot (WB), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for human, mouse and rat samples.
- Cited in over 135 publications
- Tried and trusted by researchers since 2006
- Affinity purified
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- iScience 26:1083062023Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei WangPubMed 38026209
- Nature 621:373-3802023Single-cell brain organoid screening identifies developmental defects in autism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Li,Jonas Simon Fleck,Catarina Martins-Costa,Thomas R Burkard,Jan Themann,Marlene Stuempflen,Angela Maria Peer,Ábel Vertesy,Jamie B Littleboy,Christopher Esk,Ulrich Elling,Gregor Kasprian,Nina S Corsini,Barbara Treutlein,Juergen A KnoblichPubMed 37704762
- eLife 12:2023Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Rupendra Shrestha,Albert Lowe,Xusheng Zhang,Ludovic SpaethPubMed 37665325
- Molecular neurobiology 60:7297-73082023Deletion of TRPC6, an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyung Chul Shin,Gowher Ali,Houda Yasmine Ali Moussa,Vijay Gupta,Alberto de la Fuente,Hyung-Goo Kim,Lawrence W Stanton,Yongsoo ParkPubMed 37552395
- Nature protocols 18:1893-19292023Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElke Gabriel,Walid Albanna,Giovanni Pasquini,Anand Ramani,Natasa Josipovic,Aruljothi Mariappan,Maria Giovanna Riparbelli,Giuliano Callaini,Celeste M Karch,Olivier Goureau,Argyris Papantonis,Volker Busskamp,Toni Schneider,Jay GopalakrishnanPubMed 37198320
- STAR protocols 4:1022662023Reproducible and scalable differentiation of highly pure cortical neurons from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngelika Dannert,Julien Klimmt,Carolina Cardoso Gonçalves,Dennis Crusius,Dominik PaquetPubMed 37148244
- Frontiers in molecular neuroscience 16:10645792023Role and mechanism of FOXG1-related epigenetic modifications in cisplatin-induced hair cell damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Rong Mu,Sheng-Yu Zou,Ming Li,Yan-Yan Ding,Xiang Huang,Zu-Hong He,Wei-Jia KongPubMed 37181652
- Frontiers in cellular neuroscience 17:11144202023A simplified protocol for the generation of cortical brain organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristel N Eigenhuis,Hedda B Somsen,Mark van der Kroeg,Hilde Smeenk,Anne L Korporaal,Steven A Kushner,Femke M S de Vrij,Debbie L C van den BergPubMed 37082206
- Brain communications 5:fcac3212023The PSEN1 E280G mutation leads to increased amyloid-β43 production in induced pluripotent stem cell neurons and deposition in brain tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNanet Willumsen,Charles Arber,Christopher Lovejoy,Jamie Toombs,Argyro Alatza,Philip S J Weston,Lucia Chávez-Gutiérrez,John Hardy,Henrik Zetterberg,Nick C Fox,Natalie S Ryan,Tammaryn Lashley,Selina WrayPubMed 36687397
- Brain, behavior and evolution 98:61-752022Molecular Diversity of Neuron Types in the Salamander Amygdala and Implications for Amygdalar Evolution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAstrid Deryckere,Jamie Woych,Eliza C B Jaeger,Maria Antonietta ToschesPubMed 36574764
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
Alternative names
FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3
Recommended products
Anti-FOXG1 antibody is a rabbit polyclonal antibody that is used in forkhead box protein G1 (FOXG1) western blot (WB), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for human, mouse and rat samples.
- Cited in over 135 publications
- Tried and trusted by researchers since 2006
- Affinity purified
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab18259 are tested in IHC-P. Previous batches were additionally validated in WB. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab196868.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-FOXG1 antibody (ab18259) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in firefly samples.
Anti-FOXG1 antibody (ab18259) has been cited over 140 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-FOXG1 antibody (ab18259) has high sensitivity and specificity.
Anti-FOXG1 antibody (ab18259) has 13 independent reviews from customers.
Anti-FOXG1 antibody (ab18259) specifically detects FOXG1 (UniProt ID: P55316; Molecular weight: 52kDa) and is sold in 100 µg selling sizes.
FOXG1 is a critical transcription factor involved in brain development and is associated with various neurological disorders. Research indicates that mutations in FOXG1 can lead to severe developmental issues, making it a significant target for gene therapy. Understanding FOXG1's role can provide insights into potential treatments for conditions like Rett syndrome syndrome.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.
- Biological function summary
FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.
- Pathways
FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.
- Associated diseases and disorders
FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody (ab18259)
IHC image of FoxG1 staining in mouse brain E14 formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18259, 0.5 μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining revealed in telencephalon (but not diencephalon) as expected.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-FOXG1 antibody (ab18259)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-FOXG1 antibody (ab18259) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: E10 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 4: Brain (Rat) Tissue Lysate at 10 µg
Lane 5: Human spleen tissue lysate - total protein (negative control) at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 155 kDa, 50 kDa, 52 kDa
Exposure time: 3min
Western blot - Anti-FOXG1 antibody (ab18259)
ab18259 detects a band of ~50kDa in brain lysates. This band is blocked by addition of the immunizing peptide ab19644 which suggests that is specific for FOXG1.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (Goat Anti-Rabbit IgG H&L (HRP) ab97051), and visualised using ECL development solution.
All lanes: Western blot - Anti-FOXG1 antibody (ab18259) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: Brain (Rat) Tissue Lysate at 10 µg
Lane 4: Human brain tissue lysate - total protein (ab29466) at 10 µg with Human FOXG1 peptide (ab19644)
Lane 5: Brain (Mouse) Tissue Lysate at 10 µg with Human FOXG1 peptide (ab19644)
Lane 6: Brain (Rat) Tissue Lysate at 10 µg with Human FOXG1 peptide (ab19644)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1min
This image is courtesy of Vassiliki Fotaki, University of EdinburghImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody (ab18259)
ab18259 staining Foxg1 (1/50) in the telencephalon (but not the diencephalon) as expected. DAB-immunohistochemistry was performed on embryonic (E13.5) mouse brain (coronal paraffin-embedded sections) after microwave treatment with 10mM sodium citrate.
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