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AB3394

Anti-FOXG1 antibody

1

(1 Review)

|

(5 Publications)

Goat Polyclonal FOXG1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FOXG1 aa 450 to C-terminus.

View Alternative Names

FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3

3 Images
Western blot - Anti-FOXG1 antibody (AB3394)
  • WB

Supplier Data

Western blot - Anti-FOXG1 antibody (AB3394)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-FOXG1 antibody (ab3394) at 0.5 µg/mL

Lane 1:

Human Cerebellum tissue lysate at 35 µg

Lane 2:

Cerebral Cortex tissue lysate at 35 µg

Predicted band size: 54 kDa

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Western blot - Anti-FOXG1 antibody (AB3394)
  • WB

Supplier Data

Western blot - Anti-FOXG1 antibody (AB3394)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-FOXG1 antibody (ab3394) at 1 µg/mL

All lanes:

U251 cell lysate at 35 µg

Predicted band size: 54 kDa

false

Flow Cytometry - Anti-FOXG1 antibody (AB3394)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FOXG1 antibody (AB3394)

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human FOXG1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P55316

Specificity

This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.</p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.
Biological function summary

FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.

Pathways

FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.

FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
See full target information FOXG1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:2565-2577 PubMed38932521

2024

FoxG1/BNIP3 axis promotes mitophagy and blunts cisplatin resistance in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Baolong Pan,Yan Li,Huiyun Han,Lu Zhang,Xuemei Hu,Yanyu Pan,Zhuohui Peng

CNS neuroscience & therapeutics 29:1785-1804 PubMed36880283

2023

Transplantation of glutamatergic neuronal precursor cells in the paraventricular thalamus and claustrum facilitates awakening with recovery of consciousness.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhao,Naili Wei,Tianwen Li,Kezhu Chen,Wenqiang Cui,Zhifu Wang,Fan Wang,Yuanxiang Lin,Jianhong Zhu

Molecular psychiatry 28:497-514 PubMed35318461

2022

Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Schäffner,Marie-Theres Wittmann,Tanja Vogel,D Chichung Lie

Molecular endocrinology (Baltimore, Md.) 27:1113-27 PubMed23660594

2013

Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan V Li,Christopher D Chien,Jason P Garee,Jianming Xu,Anton Wellstein,Anna T Riegel

Nature cell biology 9:531-40 PubMed17435750

2007

The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF.

Applications

ICC/IF

Species

Mouse

Tarik Regad,Martin Roth,Nicholas Bredenkamp,Nicola Illing,Nancy Papalopulu
View all publications
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Product promise

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