Goat Polyclonal FOXG1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FOXG1 aa 450 to C-terminus.
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Publications
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- Molecular psychiatry 28:497-5142022Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIris Schäffner,Marie-Theres Wittmann,Tanja Vogel,D Chichung LiePubMed 35318461
- Molecular endocrinology (Baltimore, Md.) 27:1113-272013Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan V Li et. al.PubMed 23660594
- Nature cell biology 9:531-402007The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FOXG1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55316
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Reactivity data
Select an application| Flow Cyt | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.3-1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
Alternative names
FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3
Recommended products
Goat Polyclonal FOXG1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FOXG1 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FOXG1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55316
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.
- Biological function summary
FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.
- Pathways
FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.
- Associated diseases and disorders
FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Flow Cytometry - Anti-FOXG1 antibody (ab3394)
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-FOXG1 antibody (ab3394)
Detected by chemiluminescence.
All lanes: Western blot - Anti-FOXG1 antibody (ab3394) at 1 µg/mL
All lanes: U251 cell lysate at 35 µg
Predicted band size: 54 kDa
Western blot - Anti-FOXG1 antibody (ab3394)
Detected by chemiluminescence.
All lanes: Western blot - Anti-FOXG1 antibody (ab3394) at 0.5 µg/mL
Lane 1: Human Cerebellum tissue lysate at 35 µg
Lane 2: Cerebral Cortex tissue lysate at 35 µg
Predicted band size: 54 kDa
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