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Anti-FOXG1 antibody [EPR18987] is a rabbit monoclonal antibody that is used in FOXG1 western blot (WB), IHC, immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP) and flow cytometry. Suitable for human, mouse and rat samples.


- Recombinant format for unrivaled batch-batch consistency
- Antibody clone EPR18987 is the most widely used clone for FOXG1 on the market
- One antibody for all your FOXG1 staining

Images

Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (AB196868), expandable thumbnail
  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (AB196868), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (AB196868), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (AB196868), expandable thumbnail
  • Western blot - Anti-FOXG1 antibody [EPR18987] (AB196868), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Expected
Expected
Expected
Tested
Mouse
Tested
Tested
Expected
Tested
Expected
Tested
Rat
Expected
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/60
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

For mouse and rat samples we recommend a dilution of 1/2000.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

For mouse and rat samples we recommend a dilution of 1/2000.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

For mouse and rat samples we recommend a dilution of 1/2000.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

Alternative names

FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3

Recommended products

Anti-FOXG1 antibody [EPR18987] is a rabbit monoclonal antibody that is used in FOXG1 western blot (WB), IHC, immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP) and flow cytometry. Suitable for human, mouse and rat samples.


- Recombinant format for unrivaled batch-batch consistency
- Antibody clone EPR18987 is the most widely used clone for FOXG1 on the market
- One antibody for all your FOXG1 staining

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18987
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.

Biological function summary

FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.

Pathways

FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.

Associated diseases and disorders

FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868)

    FOXG1 was immunoprecipitated from 0.35 mg mouse brain lysate 10 μg with ab196868 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab196868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: mouse brain lysate 10 μg

    Lane 2: ab196868 IP in mouse brain cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab196868 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32s

    All lanes: Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  • Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID: 26508630). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

    Lane 1: Rat E18 brain lysate at 20 µg

    Lane 2: Mouse E12.5 brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa

    Observed band size: 58 kDa

    Exposure time: 3s

  • Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Intracellular flow cytometric analysis of4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line)cell line labeling FOXG1 with ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  • Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868), expandable thumbnail

    Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lanes 1-4: 15 seconds; Lanes 5-12: 3 minutes.

    This target is expressed during brain development (PMID: 26896590, PMID: 7959731). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human fetal spleen lysate at 10 µg

    Lane 5: Mouse brain lysate at 10 µg

    Lane 6: Mouse heart lysate at 10 µg

    Lane 7: Mouse kidney lysate at 10 µg

    Lane 8: Mouse spleen lysate at 10 µg

    Lane 9: Rat brain lysate at 10 µg

    Lane 10: Rat heart lysate at 10 µg

    Lane 11: Rat liver lysate at 10 µg

    Lane 12: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa

    Observed band size: 58 kDa

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Product protocols

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