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Rabbit Recombinant Monoclonal FOXG1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888), expandable thumbnail
  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Predicted
Expected
Predicted
Predicted
Predicted
Predicted
Mouse
Tested
Expected
Expected
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

8 products for Alternative Version

Target data

Function

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

Alternative names

FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3

Recommended products

Rabbit Recombinant Monoclonal FOXG1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18987
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab227888 is the carrier-free version of Anti-FOXG1 antibody [EPR18987] ab196868.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.

Biological function summary

FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.

Pathways

FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.

Associated diseases and disorders

FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    FOXG1 was immunoprecipitated from 0.35 mg mouse brain lysate 10 μg with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FOXG1 antibody [EPR18987] ab196868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: mouse brain lysate 10 μg

    Lane 2: Anti-FOXG1 antibody [EPR18987] ab196868 IP in mouse brain cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FOXG1 antibody [EPR18987] ab196868 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32s

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

    All lanes: Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (Anti-FOXG1 antibody [EPR18987] ab196868)

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

  • Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line) cell line labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with Anti-FOXG1 antibody [EPR18987] ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXG1 antibody [EPR18987] ab196868).

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Product protocols

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