AB229241

Anti-FOXH1 antibody

Name: Anti-FOXH1 antibody (ab229241) | Abcam
Brand: Abcam Limited
SKU: AB229241

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(2 Publications)

Rabbit Polyclonal FOXH1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FOXH1.

View Alternative Names

FAST1, FAST2, FOXH1, Forkhead box protein H1, Forkhead activin signal transducer 1, Forkhead activin signal transducer 2, Fast-1, hFAST-1, Fast-2

2 Images

Supplier Data

Western blot - Anti-FOXH1 antibody (AB229241)

10% SDS-PAGE

All lanes:

Western blot - Anti-FOXH1 antibody (ab229241) at 1/500 dilution

Lane 1:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Lane 2:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 39 kDa

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Supplier Data

Western blot - Anti-FOXH1 antibody (AB229241)

10% SDS-PAGE

All lanes:

Western blot - Anti-FOXH1 antibody (ab229241) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

NCI-H1299 whole cell lysate at 30 µg

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human FOXH1. The exact immunogen used to generate this antibody is proprietary information.

O75593

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor FOXH1 also known as Forkhead Box H1 FOXH1a or Fast-1 plays a significant mechanical role in gene regulation. With a mass of approximately 45 kDa FOXH1 is expressed in various tissues including embryonic and adult tissues. It particularly localizes in the nucleus reflecting its function in modulating the expression of target genes through DNA binding.

Biological function summary

FOXH1 functions as an essential component in cell signaling by acting as a transcriptional activator. It interacts with other proteins as part of the transforming growth factor-beta (TGF-β) signaling pathway to influence cell fate determination and development. FOXH1 operates within complexes partnering frequently with SMAD2 and SMAD3 to effectively mediate cellular responses to extracellular signals.

Pathways

FOXH1 occupies a critical position within the TGF-β signaling and Nodal signaling pathways. These pathways are deeply involved in embryonic patterning and the regulation of cell cycle progression. FOXH1 interacts with proteins such as SMAD2 SMAD4 and NODAL which work together to activate specific gene sets essential for embryonic development and tissue homeostasis.

FOXH1 links with conditions rooted in developmental defects and cancer. Mutations or dysregulation in FOXH1 expression associate with congenital disorders affecting the heart and other organs. Also its interaction with proteins like NODAL and SMAD factors implicates it in the progression of certain cancers by promoting aberrant cell proliferation and differentiation. Understanding FOXH1's role in these processes aids in the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

See full target information FOXH1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1711 PubMed39739005

2025

CAF-EVs carry lncRNA MAPKAPK5-AS1 into hepatocellular carcinoma cells and promote malignant cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Sheng,Junmei Lin,Yili Zhang,Yanping Chen,Xuxing Ye,Xiaobo Wang

Frontiers in pharmacology 13:857774 PubMed35592424

2022

Comprehensive Analysis of the Potential Immune-Related Biomarker ATG101 that Regulates Apoptosis of Cholangiocarcinoma Cells After Photodynamic Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Jian Zhang,Kun-Peng Wang,Yun-Peng Huang,Chong Jin,Hao Jiang,Li Xiong,Zhao-Yi Chen,Yu Wen,Zhong-Tao Liu,Jing-Gang Mo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXH1 antibody

Western blot - Anti-FOXH1 antibody (AB229241)

Western blot - Anti-FOXH1 antibody (AB229241)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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