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Rabbit Polyclonal FOXH1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FOXH1.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human FOXH1. The exact immunogen used to generate this antibody is proprietary information. Database link O75593
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Target data

Function

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Alternative names

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Rabbit Polyclonal FOXH1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FOXH1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human FOXH1. The exact immunogen used to generate this antibody is proprietary information. Database link O75593
Purification technique

Affinity purification Immunogen

Specificity

ab189960 is predicted to not cross-react with any other members of the forkhead box family.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

FOXH1 functions as an essential component in cell signaling by acting as a transcriptional activator. It interacts with other proteins as part of the transforming growth factor-beta (TGF-β) signaling pathway to influence cell fate determination and development. FOXH1 operates within complexes partnering frequently with SMAD2 and SMAD3 to effectively mediate cellular responses to extracellular signals.

Activity summary

The transcription factor FOXH1 also known as Forkhead Box H1 FOXH1a or Fast-1 plays a significant mechanical role in gene regulation. With a mass of approximately 45 kDa FOXH1 is expressed in various tissues including embryonic and adult tissues. It particularly localizes in the nucleus reflecting its function in modulating the expression of target genes through DNA binding.

Pathways

FOXH1 occupies a critical position within the TGF-β signaling and Nodal signaling pathways. These pathways are deeply involved in embryonic patterning and the regulation of cell cycle progression. FOXH1 interacts with proteins such as SMAD2 SMAD4 and NODAL which work together to activate specific gene sets essential for embryonic development and tissue homeostasis.

Associated diseases and disorders

FOXH1 links with conditions rooted in developmental defects and cancer. Mutations or dysregulation in FOXH1 expression associate with congenital disorders affecting the heart and other organs. Also its interaction with proteins like NODAL and SMAD factors implicates it in the progression of certain cancers by promoting aberrant cell proliferation and differentiation. Understanding FOXH1's role in these processes aids in the development of targeted therapeutic strategies.

Product promise

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3 product images

  • Western blot - Anti-FOXH1 antibody - N-terminal (ab189960), expandable thumbnail

    Western blot - Anti-FOXH1 antibody - N-terminal (ab189960)

    All lanes: Western blot - Anti-FOXH1 antibody - N-terminal (AB189960) at 1 µg/mL

    All lanes: human liver tissue lysate

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (ab189960), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (ab189960)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling FOXH1 with ab189960 at 5 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (ab189960), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (ab189960)

    Immunofluorescence analysis of human liver tissue labeling FOXH1 with ab189960 at 20 μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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