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AB189960

Anti-FOXH1 antibody - N-terminal

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(3 Publications)

Rabbit Polyclonal FOXH1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FOXH1.

View Alternative Names

FAST1, FAST2, FOXH1, Forkhead box protein H1, Forkhead activin signal transducer 1, Forkhead activin signal transducer 2, Fast-1, hFAST-1, Fast-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (AB189960)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (AB189960)

Immunofluorescence analysis of human liver tissue labeling FOXH1 with ab189960 at 20 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (AB189960)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXH1 antibody - N-terminal (AB189960)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling FOXH1 with ab189960 at 5 μg/ml.

Western blot - Anti-FOXH1 antibody - N-terminal (AB189960)
  • WB

Supplier Data

Western blot - Anti-FOXH1 antibody - N-terminal (AB189960)

All lanes:

Western blot - Anti-FOXH1 antibody - N-terminal (ab189960) at 1 µg/mL

All lanes:

human liver tissue lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FOXH1. The exact immunogen used to generate this antibody is proprietary information.

O75593

Specificity

ab189960 is predicted to not cross-react with any other members of the forkhead box family.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor FOXH1 also known as Forkhead Box H1 FOXH1a or Fast-1 plays a significant mechanical role in gene regulation. With a mass of approximately 45 kDa FOXH1 is expressed in various tissues including embryonic and adult tissues. It particularly localizes in the nucleus reflecting its function in modulating the expression of target genes through DNA binding.
Biological function summary

FOXH1 functions as an essential component in cell signaling by acting as a transcriptional activator. It interacts with other proteins as part of the transforming growth factor-beta (TGF-β) signaling pathway to influence cell fate determination and development. FOXH1 operates within complexes partnering frequently with SMAD2 and SMAD3 to effectively mediate cellular responses to extracellular signals.

Pathways

FOXH1 occupies a critical position within the TGF-β signaling and Nodal signaling pathways. These pathways are deeply involved in embryonic patterning and the regulation of cell cycle progression. FOXH1 interacts with proteins such as SMAD2 SMAD4 and NODAL which work together to activate specific gene sets essential for embryonic development and tissue homeostasis.

FOXH1 links with conditions rooted in developmental defects and cancer. Mutations or dysregulation in FOXH1 expression associate with congenital disorders affecting the heart and other organs. Also its interaction with proteins like NODAL and SMAD factors implicates it in the progression of certain cancers by promoting aberrant cell proliferation and differentiation. Understanding FOXH1's role in these processes aids in the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
See full target information FOXH1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging 14:2268-2286 PubMed35255005

2022

A comprehensive analysis of FOX family in HCC and experimental evidence to support the oncogenic role of FOXH1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiwu Ouyang,Lemeng Feng,Lei Yao,Jingyu Zhang,Yao Xiao,Guodong Liu,Gewen Zhang,Zhiming Wang

OncoTargets and therapy 14:1487-1499 PubMed33679133

2021

FAST1 Predicts Poor Survival of Renal Carcinoma and Promotes Its Progression Through the TGF-β/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Tian,Xiangyang Fu,Liangliang Hu,Xiaofeng Yang,Peng Sun,Fengfeng Sun

Nature 579:427-432 PubMed32132707

2020

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Guo,Giovanni Aviles,Yi Liu,Ruilin Tian,Bret A Unger,Yu-Hsiu T Lin,Arun P Wiita,Ke Xu,M Almira Correia,Martin Kampmann
View all publications
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Product promise

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