AB20454

Anti-FOXI1 antibody

Name: Anti-FOXI1 antibody. Goat polyclonal (ab20454) | Abcam
Brand: Abcam Limited
SKU: AB20454
Rating: 5 (1 reviews)

(1 Review)

(18 Publications)

Goat Polyclonal FOXI1 antibody. Suitable for ChIP, WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human FOXI1 aa 350 to C-terminus.

View Alternative Names

FKHL10, FREAC6, FOXI1, Forkhead box protein I1, Forkhead-related protein FKHL10, Forkhead-related transcription factor 6, Hepatocyte nuclear factor 3 forkhead homolog 3, FREAC-6, HFH-3, HNF-3/fork-head homolog 3

1 Images

Supplier Data

Western blot - Anti-FOXI1 antibody (AB20454)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-FOXI1 antibody (ab20454) at 1 µg/mL

All lanes:

Human Testes lysate (35µg protein in RIPA buffer)

Predicted band size: 41 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IHC-Fr, ChIP, WB

applications

Immunogen

Synthetic Peptide within Human FOXI1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12951

Specificity

This antibody is expected to recognise both reported human isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Primary incubation 1 hour at room temperature.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "An antigen retrieval method using SDS is described in PubMed 16932748 and PubMed 9072183." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Forkhead box I1 (FOXI1) also known by various alternative names such as HFH3 or FKHL10 is a transcription factor with a mass of approximately 40 kDa. It is predominantly expressed in epithelial tissues including the kidney and inner ear. FOXI1 plays a mechanical role by binding to specific DNA sequences to regulate the transcription of target genes influencing cellular development and differentiation.

Biological function summary

FOXI1 impacts several fundamental processes including the development of epithelial structures. It acts as a member of the forkhead family of transcription factors known for their roles in cellular growth and differentiation. FOXI1 does not typically form part of a larger protein complex but it interacts with other proteins such as FOXE1 and FGFR2 to execute its biological functions efficiently. These interactions are important for proper organ formation and function.

Pathways

Several cellular and developmental pathways involve FOXI1. Notably FOXI1 plays an important role in the Wnt signaling pathway essential for cell fate determination and organ development. In this context FOXI1 regulates gene expression by interacting with other pathway components like β-catenin. Additionally FOXI1 participates in the hedgehog signaling pathway where its involvement influences gene transcription in response to developmental cues.

Mutations or dysregulation of FOXI1 have connections to conditions such as distal renal tubular acidosis and sensorineural hearing loss. In distal renal tubular acidosis FOXI1's transcriptional control affects ion channel and transporter expression in the kidney. The disorder links to other proteins like AE1 and H+-ATPase where altered FOXI1 activity influences their expression or function. Sensorineural hearing loss associations with FOXI1 involve its regulatory roles in the inner ear's development affecting auditory function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules (By similarity).

See full target information FOXI1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadm8663 PubMed39383236

2024

SLC26A4-AP-2 mu2 interaction regulates SLC26A4 plasma membrane abundance in the endolymphatic sac.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Jae Lee,Cristina Fenollar-Ferrer,Kevin Isgrig,Ya-Xian Wang,Kerstin Valente,Juleh Eide,Keiji Honda,Wade W Chien,Ronald S Petralia,Lijin Dong,Thomas B Friedman,Juan S Bonifacino,Andrew J Griffith,Isabelle Roux

Journal of the American Society of Nephrology : JASN 35:533-548 PubMed38332484

2024

Foxp1 Is Required for Renal Intercalated Cell Differentiation and Acid-Base Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Ting Wu,Yu Feng,Renhua Song,Yanmiao Qi,Lin Li,Dongbo Lu,Yixuan Wang,Wenrun Wu,Angela Morgan,Xiaohong Wang,Yin Xia,Renjing Liu,Stephen I Alexander,Justin Wong,Yuzhen Zhang,Xiangjian Zheng

Nature 621:857-867 PubMed37730992

2023

Transgenic ferret models define pulmonary ionocyte diversity and function.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yuan,Grace N Gasser,Evan Lemire,Daniel T Montoro,Karthik Jagadeesh,Yan Zhang,Yifan Duan,Vitaly Ievlev,Kristen L Wells,Pavana G Rotti,Weam Shahin,Michael Winter,Bradley H Rosen,Idil Evans,Qian Cai,Miao Yu,Susan A Walsh,Michael R Acevedo,Darpan N Pandya,Vamsidhar Akurathi,David W Dick,Thaddeus J Wadas,Nam Soo Joo,Jeffrey J Wine,Susan Birket,Courtney M Fernandez,Hui Min Leung,Guillermo J Tearney,Alan S Verkman,Peter M Haggie,Kathleen Scott,Douglas Bartels,David K Meyerholz,Steven M Rowe,Xiaoming Liu,Ziying Yan,Adam L Haber,Xingshen Sun,John F Engelhardt

Human genetics 142:1499-1517 PubMed37668839

2023

CHD7 variants associated with hearing loss and enlargement of the vestibular aqueduct.

Applications

Unspecified application

Species

Unspecified reactive species

Isabelle Roux,Cristina Fenollar-Ferrer,Hyun Jae Lee,Parna Chattaraj,Ivan A Lopez,Kyungreem Han,Keiji Honda,Carmen C Brewer,John A Butman,Robert J Morell,Donna M Martin,Andrew J Griffith

EBioMedicine 92:104596 PubMed37182269

2023

Comparative analyses define differences between BHD-associated renal tumour and sporadic chromophobe renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Jikuya,Todd A Johnson,Kazuhiro Maejima,Jisong An,Young-Seok Ju,Hwajin Lee,Kyungsik Ha,WooJeung Song,Youngwook Kim,Yuki Okawa,Shota Sasagawa,Yuki Kanazashi,Masashi Fujita,Seiya Imoto,Taku Mitome,Shinji Ohtake,Go Noguchi,Sachi Kawaura,Yasuhiro Iribe,Kota Aomori,Tomoyuki Tatenuma,Mitsuru Komeya,Hiroki Ito,Yusuke Ito,Kentaro Muraoka,Mitsuko Furuya,Ikuma Kato,Satoshi Fujii,Haruka Hamanoue,Tomohiko Tamura,Masaya Baba,Toshio Suda,Tatsuhiko Kodama,Kazuhide Makiyama,Masahiro Yao,Brian M Shuch,Christopher J Ricketts,Laura S Schmidt,W Marston Linehan,Hidewaki Nakagawa,Hisashi Hasumi

American journal of physiology. Renal physiology 324:F267-F273 PubMed36603001

2023

The transcription factor Foxi1 promotes expression of V-ATPase and Gpr116 in M-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mackenzie Kui,Jennifer L Pluznick,Nathan A Zaidman

Molecular therapy. Methods & clinical development 26:371-383 PubMed36034771

2022

AAV8BP2 and AAV8 transduce the mammalian cochlear lateral wall and endolymphatic sac with high efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Isgrig,Yasuko Ishibashi,Hyun Jae Lee,Jianliang Zhu,Mhamed Grati,Jean Bennett,Andrew J Griffith,Isabelle Roux,Wade W Chien

Clinical and translational medicine 12:e747 PubMed35220675

2022

Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Xue,Lifang Ma,Xiao Zhang,Xin Xu,Susu Guo,Yikun Wang,Shiyu Qiu,Jiangtao Cui,Wanxin Guo,Yongchun Yu,Fenyong Sun,Yi Shi,Jiayi Wang

Nature 560:319-324 PubMed30069044

2018

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel T Montoro,Adam L Haber,Moshe Biton,Vladimir Vinarsky,Brian Lin,Susan E Birket,Feng Yuan,Sijia Chen,Hui Min Leung,Jorge Villoria,Noga Rogel,Grace Burgin,Alexander M Tsankov,Avinash Waghray,Michal Slyper,Julia Waldman,Lan Nguyen,Danielle Dionne,Orit Rozenblatt-Rosen,Purushothama Rao Tata,Hongmei Mou,Manjunatha Shivaraju,Hermann Bihler,Martin Mense,Guillermo J Tearney,Steven M Rowe,John F Engelhardt,Aviv Regev,Jayaraj Rajagopal

Nature 560:377-381 PubMed30069046

2018

A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey W Plasschaert,Rapolas Žilionis,Rayman Choo-Wing,Virginia Savova,Judith Knehr,Guglielmo Roma,Allon M Klein,Aron B Jaffe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXI1 antibody

Western blot - Anti-FOXI1 antibody (AB20454)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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