Goat Polyclonal FOXI3 antibody. Suitable for WB and reacts with Mouse, Rat samples.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link D3Z120
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-3 µg/mL | Notes Primary incubation time: 1 hour. |
Species Rat | Dilution info 1-3 µg/mL | Notes Primary incubation time: 1 hour. |
Target data
Function
Transcription factor required for pharyngeal arch development, which is involved in hair, ear, jaw and dental development (PubMed:24650709, PubMed:26550799, PubMed:26992132, PubMed:30467319). May act as a pioneer transcription factor during pharyngeal arch development (PubMed:26550799, PubMed:26992132). Required for epithelial cell differentiation within the epidermis (PubMed:26450968). Acts at multiple stages of otic placode induction: necessary for preplacodal ectoderm to execute an inner ear program (PubMed:26550799). Required for hair follicle stem cell specification (PubMed:26992132). Acts downstream of TBX1 for the formation of the thymus and parathyroid glands from the third pharyngeal pouch (PubMed:31412026).
Alternative names
Forkhead box protein I3
Recommended products
Goat Polyclonal FOXI3 antibody. Suitable for WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link D3Z120
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab113424 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target protein FOXI3 also known as Forkhead box protein I3 functions as a transcription factor involved in regulating gene expression. It has a mass of approximately 43 kDa. FOXI3 is mainly expressed in tissues derived from the ectoderm such as skin hair follicles and tooth buds. This expression pattern suggests a role in the development and differentiation of these tissues.
- Biological function summary
The FOXI3 protein plays a central role in the development of ectodermal structures. It does not form part of a larger protein complex but operates by binding to DNA and controlling the expression of genes necessary for the development and morphogenesis of hair and dental structures. Its regulation of these developmental processes highlights its importance in proper ectodermal tissue function.
- Pathways
The FOXI3 protein participates in the WNT signaling pathway which is important for cell differentiation and proliferation in ectodermal tissues. It also interacts with other transcription factors such as Lef1 to regulate hair follicle development. The interplay between FOXI3 and these proteins highlights its involvement in pathways that determine ectodermal tissue fate.
- Associated diseases and disorders
Genetic mutations in FOXI3 have been linked to ectodermal dysplasia a condition characterized by abnormal development of the skin hair and teeth. Research has shown that mutations can disrupt its usual function leading to these developmental issues. Additionally FOXI3 shows a connection with the protein MSX1 which also influences tooth development further implicating its role in dental disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-FOXI3 antibody (ab113424)
Detected by chemiluminescence.
All lanes: Western blot - Anti-FOXI3 antibody (ab113424) at 1 µg/mL
All lanes: Rat Skin lysate (35µg protein in RIPA buffer)
Predicted band size: 43 kDa
Western blot - Anti-FOXI3 antibody (ab113424)
All lanes: Western blot - Anti-FOXI3 antibody (ab113424) at 1 µg/mL
All lanes: Mouse Skin lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Downloads
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