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AB220028

Anti-FoxJ1 antibody [CL3989]

2

(1 Review)

|

(3 Publications)

Mouse Monoclonal FOXJ1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FOXJ1 aa 150-350.

View Alternative Names

FKHL13, HFH4, FOXJ1, Forkhead box protein J1, Forkhead-related protein FKHL13, Hepatocyte nuclear factor 3 forkhead homolog 4, HFH-4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.

Immunocytochemistry - Anti-FoxJ1 antibody [CL3989] (AB220028)
  • ICC

Supplier Data

Immunocytochemistry - Anti-FoxJ1 antibody [CL3989] (AB220028)

Immunocytochemistry/ Immunofluorescence analysis of BEWO cells labeling FoxJ1 with ab220028 at 2-10 μg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (AB220028)

Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3989

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human FOXJ1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q92949

Epitope

Binds to an epitope located within the peptide sequence EPGKGGFWRI (aa 188-197) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FoxJ1 also known as Forkhead Box J1 is a transcription factor that plays an important role in the regulation of gene expression. It is part of the forkhead family of transcription factors and has a molecular mass of approximately 44 kDa. FoxJ1 is expressed mainly in tissues with motile cilia such as the brain respiratory tract and reproductive organs. This expression pattern highlights its significance in tissues where ciliary movement is important for normal function.
Biological function summary

FoxJ1 is important in ciliogenesis the process of building cilia on the surface of cells. It acts as a master regulator by activating genes that code for ciliary components. This includes genes involved in the formation of the axoneme the core structure of cilia. FoxJ1 does not work alone; it functions within a network of other transcription factors and proteins to achieve effective cilia formation and movement. By controlling this process FoxJ1 ensures that ciliary structures develop and function correctly which is necessary for cellular motility and fluid movement across epithelial surfaces.

Pathways

FoxJ1 operates within critical pathways involved in cilia development and function. It integrates into the Wnt signaling pathway which is critical for embryogenesis and cell polarity. Additionally FoxJ1 interacts with the Notch signaling pathway. In these pathways it can relate to other transcription factors like Rfx3 which also influence cilia-related gene expression. FoxJ1 coordinates with these pathways to ensure precise regulation of cilia biogenesis and maintenance contributing to cellular processes that depend on functional cilia.

Deficient or malfunctioning FoxJ1 can lead to primary ciliary dyskinesia (PCD) and respiratory diseases. PCD results from impaired ciliary movement affecting respiratory tract function and fertility. Dysfunctional FoxJ1 expression can disrupt ciliary architecture connecting it to other proteins like DNAI1 which is often implicated in PCD. Abnormal FoxJ1 activity has also been linked to hydrocephalus where cerebrospinal fluid imbalances occur due to faulty cilia movement. In these conditions understanding FoxJ1's role provides insight into potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor specifically required for the formation of motile cilia (PubMed : 31630787). Acts by activating transcription of genes that mediate assembly of motile cilia, such as CFAP157. Binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T). Activates the transcription of a variety of ciliary proteins in the developing brain and lung.
See full target information FOXJ1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 58:e13765 PubMed39407407

2024

Gdf11 regulates left-right asymmetry development through TGF-β signal.

Applications

Unspecified application

Species

Unspecified reactive species

Wantao Yao,Zhaohui Wei,Xinning Tian,Jin Tan,Jingwen Liu

Neural regeneration research 18:396-403 PubMed35900436

2022

Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ri-Yun Yang,Rui Chai,Jing-Ying Pan,Jing-Yin Bao,Pan-Hui Xia,Yan-Kai Wang,Ying Chen,Yi Li,Jian Wu,Gang Chen

PLoS biology 17:e3000203 PubMed31430272

2019

Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Liu,Chengke Zhu,Guozhu Ning,Liping Yang,Yu Cao,Sizhou Huang,Qiang Wang
View all publications
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