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AB238168

Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FOXJ1 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

FKHL13, HFH4, FOXJ1, Forkhead box protein J1, Forkhead-related protein FKHL13, Hepatocyte nuclear factor 3 forkhead homolog 4, HFH-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)

Immunohistochemical analysis of paraffin-embedded human pinealoma tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining in human pinealoma (PMID : 28719464) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235445).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)

Immunohistochemical analysis of paraffin-embedded human choroid plexus papilloma tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human choroid plexus papilloma (PMID : 28719464; PMID : 26690880) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab235445).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in bronchiole of rat lung (PMID : 26690880; PMID : 19502490) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235445).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [EPR21874] - BSA and Azide free (AB238168)

Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse fallopian tube (PMID : 26690880; PMID : 27562488) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235445).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21874

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab238168 is the carrier-free version of ab235445.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FoxJ1 also known as Forkhead Box J1 is a transcription factor that plays an important role in the regulation of gene expression. It is part of the forkhead family of transcription factors and has a molecular mass of approximately 44 kDa. FoxJ1 is expressed mainly in tissues with motile cilia such as the brain respiratory tract and reproductive organs. This expression pattern highlights its significance in tissues where ciliary movement is important for normal function.
Biological function summary

FoxJ1 is important in ciliogenesis the process of building cilia on the surface of cells. It acts as a master regulator by activating genes that code for ciliary components. This includes genes involved in the formation of the axoneme the core structure of cilia. FoxJ1 does not work alone; it functions within a network of other transcription factors and proteins to achieve effective cilia formation and movement. By controlling this process FoxJ1 ensures that ciliary structures develop and function correctly which is necessary for cellular motility and fluid movement across epithelial surfaces.

Pathways

FoxJ1 operates within critical pathways involved in cilia development and function. It integrates into the Wnt signaling pathway which is critical for embryogenesis and cell polarity. Additionally FoxJ1 interacts with the Notch signaling pathway. In these pathways it can relate to other transcription factors like Rfx3 which also influence cilia-related gene expression. FoxJ1 coordinates with these pathways to ensure precise regulation of cilia biogenesis and maintenance contributing to cellular processes that depend on functional cilia.

Deficient or malfunctioning FoxJ1 can lead to primary ciliary dyskinesia (PCD) and respiratory diseases. PCD results from impaired ciliary movement affecting respiratory tract function and fertility. Dysfunctional FoxJ1 expression can disrupt ciliary architecture connecting it to other proteins like DNAI1 which is often implicated in PCD. Abnormal FoxJ1 activity has also been linked to hydrocephalus where cerebrospinal fluid imbalances occur due to faulty cilia movement. In these conditions understanding FoxJ1's role provides insight into potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor specifically required for the formation of motile cilia (PubMed : 31630787). Acts by activating transcription of genes that mediate assembly of motile cilia, such as CFAP157. Binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T). Activates the transcription of a variety of ciliary proteins in the developing brain and lung.
See full target information FOXJ1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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