AB58622

Anti-FOXL2 antibody [262C1a]

Name: Anti-FOXL2 antibody [262C1a] (ab58622) | Abcam
Brand: Abcam Limited
SKU: AB58622
Price: 535 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(1 Publication)

Mouse Monoclonal FOXL2 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FOXL2 aa 100-250.

View Alternative Names

Forkhead box protein L2, FOXL2

1 Images

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Western blot - Anti-FOXL2 antibody [262C1a] (AB58622)

The low MW of the band is due to the fact that the immunising recombinant fragment, rather than the full lenght protein, was used as positive control.

All lanes:

Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)

Predicted band size: 38 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

262C1a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human FOXL2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P58012

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FOXL2 also known as Forkhead box L2 functions as a transcription factor belonging to the forkhead family. It has an approximate mass of 45 kDa. This protein is expressed mainly in ovarian somatic cells and certain tissues of the eye. FOXL2 contains a distinct forkhead domain which binds to DNA and regulates gene expression involved in cellular proliferation differentiation and apoptosis.

Biological function summary

Cells rely on FOXL2 for regulating early ovarian development and maintenance of granulosa cell identity. As part of transcriptional complexes it interacts with other proteins to maintain ovarian function and health. FOXL2 also inhibits certain genes that are otherwise potentially involved in abnormal cell growth acting as a tumor suppressor in ovarian tissues. This makes it essential in reproductive biology.

Pathways

FOXL2 plays significant roles in ovarian development and function through the TGF-beta signaling pathway and the Wnt signaling pathway. In collaboration with SMAD3 a protein in the TGF-beta pathway FOXL2 regulates genes important for cell communication and differentiation. Additionally it interfaces with proteins involved in cellular structure and movement influencing biological processes critical for tissue maintenance.

Mutations in FOXL2 are linked to disorders like Blepharophimosis Ptosis Epicantherefore Inversus Syndrome (BPES) and ovarian granulosa cell tumors. FOXL2 mutations often disrupt the protein’s interactions with other factors such as the androgen receptor leading to abnormal cellular function. In BPES FOXL2 mutations impair eyelid formation and function highlighting its role in craniofacial development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

See full target information FOXL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 11:3028-3032 PubMed27123058

2016

Suppression of human epidermal growth factor receptor 2 via interference increases the chemosensitivity of ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Song,Kailv Sun,Jianming Hu,Jianghu Zhou

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXL2 antibody [262C1a]

Western blot - Anti-FOXL2 antibody [262C1a] (AB58622)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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