Mouse Monoclonal FOXL2 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXL2 aa 100-250.
Images
![Western blot - Anti-FOXL2 antibody [262C1a] (AB58622), expandable thumbnail](https://content.abcam.com/products/images/foxl2-antibody-262c1a-ab58622--western-blot-img97071.gif "Western blot - Anti-FOXL2 antibody [262C1a] (AB58622)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FOXL2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P58012
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
Alternative names
Forkhead box protein L2, FOXL2
Recommended products
Mouse Monoclonal FOXL2 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOXL2 aa 100-250.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FOXL2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P58012
- Clone number
- 262C1a
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein FOXL2 also known as Forkhead box L2 functions as a transcription factor belonging to the forkhead family. It has an approximate mass of 45 kDa. This protein is expressed mainly in ovarian somatic cells and certain tissues of the eye. FOXL2 contains a distinct forkhead domain which binds to DNA and regulates gene expression involved in cellular proliferation differentiation and apoptosis.
- Biological function summary
Cells rely on FOXL2 for regulating early ovarian development and maintenance of granulosa cell identity. As part of transcriptional complexes it interacts with other proteins to maintain ovarian function and health. FOXL2 also inhibits certain genes that are otherwise potentially involved in abnormal cell growth acting as a tumor suppressor in ovarian tissues. This makes it essential in reproductive biology.
- Pathways
FOXL2 plays significant roles in ovarian development and function through the TGF-beta signaling pathway and the Wnt signaling pathway. In collaboration with SMAD3 a protein in the TGF-beta pathway FOXL2 regulates genes important for cell communication and differentiation. Additionally it interfaces with proteins involved in cellular structure and movement influencing biological processes critical for tissue maintenance.
- Associated diseases and disorders
Mutations in FOXL2 are linked to disorders like Blepharophimosis Ptosis Epicantherefore Inversus Syndrome (BPES) and ovarian granulosa cell tumors. FOXL2 mutations often disrupt the protein’s interactions with other factors such as the androgen receptor leading to abnormal cellular function. In BPES FOXL2 mutations impair eyelid formation and function highlighting its role in craniofacial development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)
The low MW of the band is due to the fact that the immunising recombinant fragment, rather than the full lenght protein, was used as positive control.
All lanes: Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)
Predicted band size: 38 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com