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Rabbit Recombinant Monoclonal FOXL2 antibody. Suitable for IP, WB, IHC-Fr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (AB246511), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] (AB246511), expandable thumbnail
  • Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] (AB246511), expandable thumbnail
  • Western blot - Anti-FOXL2 antibody [EPR23523-68] (AB246511), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (AB246511), expandable thumbnail

Publications

  • Cells 12:2023
    Sertoli Cell-Specific Activation of Transforming Growth Factor Beta Receptor 1 Leads to Testicular Granulosa Cell Tumor Formation.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Xin Fang,Linfeng Nie,Satwikreddy Putluri,Nan Ni,Laurent Bartholin,Qinglei Li
    PubMed 38067144
  • The Journal of biological chemistry 299:1029052023
    DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Simin Zhao,Chengzi Huang,Yajuan Yang,Weiwei Xu,Yongze Yu,Canxin Wen,Lili Cao,Fei Gao,Yingying Qin,Zi-Jiang Chen,Ting Guo,Shidou Zhao
    PubMed 36642183

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-PICC/IFFlow Cyt
Human
Expected
Not recommended
Expected
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Not recommended
Rat
Expected
Tested
Expected
Tested
Expected
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

We do not recommend this antibody for WB using human samples.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

We do not recommend this antibody for WB using human samples.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

We do not recommend this antibody for WB using human samples.

Tested
Tested

Species

Mouse

Dilution info

1/250

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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    3 products for Alternative Version

      Target data

      Function

      Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

      Alternative names

      Forkhead box protein L2, FOXL2

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      Rabbit Recombinant Monoclonal FOXL2 antibody. Suitable for IP, WB, IHC-Fr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

      Alternative names

      Forkhead box protein L2, FOXL2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR23523-68

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      The protein FOXL2 also known as Forkhead box L2 functions as a transcription factor belonging to the forkhead family. It has an approximate mass of 45 kDa. This protein is expressed mainly in ovarian somatic cells and certain tissues of the eye. FOXL2 contains a distinct forkhead domain which binds to DNA and regulates gene expression involved in cellular proliferation differentiation and apoptosis.

      Biological function summary

      Cells rely on FOXL2 for regulating early ovarian development and maintenance of granulosa cell identity. As part of transcriptional complexes it interacts with other proteins to maintain ovarian function and health. FOXL2 also inhibits certain genes that are otherwise potentially involved in abnormal cell growth acting as a tumor suppressor in ovarian tissues. This makes it essential in reproductive biology.

      Pathways

      FOXL2 plays significant roles in ovarian development and function through the TGF-beta signaling pathway and the Wnt signaling pathway. In collaboration with SMAD3 a protein in the TGF-beta pathway FOXL2 regulates genes important for cell communication and differentiation. Additionally it interfaces with proteins involved in cellular structure and movement influencing biological processes critical for tissue maintenance.

      Associated diseases and disorders

      Mutations in FOXL2 are linked to disorders like Blepharophimosis Ptosis Epicantherefore Inversus Syndrome (BPES) and ovarian granulosa cell tumors. FOXL2 mutations often disrupt the protein’s interactions with other factors such as the androgen receptor leading to abnormal cellular function. In BPES FOXL2 mutations impair eyelid formation and function highlighting its role in craniofacial development.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      11 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on granulosa cells and some other cells in mouse ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse ovary tissue labeling FOXL2 with ab246511 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Nuclear staining on mouse ovary. The nuclear counter stain is DAPI (blue).

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        FOXL2 was immunoprecipitated from 0.35 mg Mouse ovary tissue lysate with ab246511 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

        Lane 1: Mouse ovary tissue lysate 10ug

        Lane 2: ab246511 IP in Mouse ovary tissue lysate

        Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246511 in mouse ovary tissue lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 84 seconds

        All lanes: Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Predicted band size: 38 kDa

        Observed band size: 50 kDa

      • Western blot - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Western blot - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 19106105）
        Negative control: Mouse testis, Rat testis (PMID: 15059956)

        All lanes: Western blot - Anti-FOXL2 antibody [EPR23523-68] (ab246511) at 1/1000 dilution

        Lane 1: Mouse ovary tissue lysate at 20 µg

        Lane 2: Mouse testis tissue lysate at 20 µg

        Lane 3: Rat ovary tissue lysate at 20 µg

        Lane 4: Rat testis tissue lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 38 kDa

        Observed band size: 50 kDa

        Exposure time: 3min

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling FOXL2 with purified ab246511 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

        Positive staining on stroma cells in human endometrium. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling FOXL2 with purified ab246511 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

        Negative control: no staining on human testis. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on granulosa cells and some other cells in rat ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse spleen. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in NIH/3T3 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

      • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] (ab246511), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] (ab246511)

        Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblasts) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in K-562 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

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      Product protocols

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