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AB272050

Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FOXL2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Forkhead box protein L2, FOXL2

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblasts) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in K-562 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse ovary tissue labeling FOXL2 with ab246511 at 1/250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Nuclear staining on mouse ovary. The nuclear counter stain is DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on granulosa cells and some other cells in mouse ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on granulosa cells and some other cells in rat ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on mouse spleen. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in NIH/3T3 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)
  • IP

Unknown

Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (AB272050)

FOXL2 was immunoprecipitated from 0.35 mg Mouse ovary tissue lysate with ab246511 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse ovary tissue lysate 10ug

Lane 2 : ab246511 IP in Mouse ovary tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246511 in mouse ovary tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 84 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

All lanes:

Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] (<a href='/en-us/products/primary-antibodies/foxl2-antibody-epr23523-68-ab246511'>ab246511</a>)

Predicted band size: 38 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23523-68

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP, IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p>We do not recommend this antibody for WB using human samples.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We do not recommend this antibody for WB using human samples.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We do not recommend this antibody for WB using human samples.</p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

ab252050 is the carrier-free version of ab246511.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FOXL2 also known as Forkhead box L2 functions as a transcription factor belonging to the forkhead family. It has an approximate mass of 45 kDa. This protein is expressed mainly in ovarian somatic cells and certain tissues of the eye. FOXL2 contains a distinct forkhead domain which binds to DNA and regulates gene expression involved in cellular proliferation differentiation and apoptosis.
Biological function summary

Cells rely on FOXL2 for regulating early ovarian development and maintenance of granulosa cell identity. As part of transcriptional complexes it interacts with other proteins to maintain ovarian function and health. FOXL2 also inhibits certain genes that are otherwise potentially involved in abnormal cell growth acting as a tumor suppressor in ovarian tissues. This makes it essential in reproductive biology.

Pathways

FOXL2 plays significant roles in ovarian development and function through the TGF-beta signaling pathway and the Wnt signaling pathway. In collaboration with SMAD3 a protein in the TGF-beta pathway FOXL2 regulates genes important for cell communication and differentiation. Additionally it interfaces with proteins involved in cellular structure and movement influencing biological processes critical for tissue maintenance.

Mutations in FOXL2 are linked to disorders like Blepharophimosis Ptosis Epicantherefore Inversus Syndrome (BPES) and ovarian granulosa cell tumors. FOXL2 mutations often disrupt the protein’s interactions with other factors such as the androgen receptor leading to abnormal cellular function. In BPES FOXL2 mutations impair eyelid formation and function highlighting its role in craniofacial development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
See full target information FOXL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Andrologia 54:e14441 PubMed35428984

2022

Methamphetamine can induce alteration of histopathology and sex determination gene expression through the oxidative stress pathway in the testes of human post-mortem.

Applications

Unspecified application

Species

Unspecified reactive species

Arefeh Aryan,Mohammad-Amin Abdollahifar,Saied Karbalay-Doust,Mehdi Forozesh,Gholam-Reza Mahmoudiasl,Soheila Akaberi-Nasrabadi,Soghra Bahmanpour
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com