AB245309

Anti-FOXM1 antibody

Name: Anti-FOXM1 antibody (ab245309) | Abcam
Brand: Abcam Limited
SKU: AB245309
Price: 525 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal FOXM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human FOXM1 aa 700 to C-terminus.

View Alternative Names

FKHL16, HFH11, MPP2, WIN, FOXM1, Forkhead box protein M1, Forkhead-related protein FKHL16, Hepatocyte nuclear factor 3 forkhead homolog 11, M-phase phosphoprotein 2, MPM-2 reactive phosphoprotein 2, Transcription factor Trident, Winged-helix factor from INS-1 cells, HFH-11, HNF-3/fork-head homolog 11, FKHL16, HFH11, MPP2, WIN, FOXM1, Forkhead box protein M1, Forkhead-related protein FKHL16, Hepatocyte nuclear factor 3 forkhead homolog 11, M-phase phosphoprotein 2, MPM-2 reactive phosphoprotein 2, Transcription factor Trident, Winged-helix factor from INS-1 cells, HFH-11, HNF-3/fork-head homolog 11

2 Images

Supplier Data

Immunoprecipitation - Anti-FOXM1 antibody (AB245309)

FOXM1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245309 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab245309 at 1 μg/ml.

Lane 1 : ab245309 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-FOXM1 antibody (ab245309)

Predicted band size: 84 kDa

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Supplier Data

Western blot - Anti-FOXM1 antibody (AB245309)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FOXM1 antibody (ab245309) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 84 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FOXM1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08050

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245309 was affinity purified using an epitope specific to FOXM1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXM1 also known as Forkhead Box M1 functions mechanically as a transcription factor. This target plays an important role in the regulation of gene expression during the cell cycle. It is characterized by a molecular weight of approximately 85 kDa. FOXM1 is mainly expressed in actively proliferating cells including the liver lungs and thymus tissues linking it with processes involved in cellular growth and division.

Biological function summary

The transcription factor FOXM1 regulates the expression of genes important for DNA replication and mitosis. It forms part of a complex network with other proteins to initiate and sustain cell proliferation. This protein regulates key regulatory genes such as Cyclin B1 and Plk1 ensuring proper transition through various phases of the cell cycle. The presence of FOXM1 in normal tissues emphasizes its role in maintaining healthy cell division.

Pathways

FOXM1 is integral to the regulation of cell cycle pathways and interacts with proteins involved in the PI3K/AKT signaling pathway. This target coordinates with various proteins to facilitate progression through cell cycle checkpoints and modulate responses to growth signals. Proteins such as CDK1 and SKP2 operate alongside FOXM1 to advance cells from G2 phase into mitosis demonstrating its involvement in cell cycle control mechanics.

FOXM1 shows significant association with cancer progression and metastasis. Overexpression of FOXM1 has been observed in colorectal and breast cancers indicating its potential as a therapeutic target. FOXM1 interacts with proteins like β-catenin in cancerous states influencing tumor growth and survival. Mutations and dysregulation of FOXM1 contribute to oncogenic processes further underlining its role in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed : 19160488, PubMed : 20360045). Plays a role in the control of cell proliferation (PubMed : 19160488). Also plays a role in DNA break repair, participating in the DNA damage checkpoint response (PubMed : 17101782). Promotes transcription of PHB2 (PubMed : 33754036).

See full target information FOXM1

Additional targets

FOXM1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical biochemistry and nutrition 77:10-17 PubMed40777817

2025

FOXM1 transcriptionally activates NEIL3 to inhibit ferroptosis in lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hailang Hou,Xinpu Geng,Xingxing Shao,Jindao Wang,Wan Xia,Huijie Chen

Hereditas 162:104 PubMed40514721

2025

Astragaloside IV regulates FOXM1 deubiquitination to ameliorate trophoblast damage caused by high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Li,Xiaofang Zhao

Cell division 20:1 PubMed39825314

2025

Interaction of STIL with FOXM1 regulates SF3A3 transcription in the hepatocellular carcinoma development.

Applications

Unspecified application

Species

Unspecified reactive species

Haijun Zhang,Lin Zhang,Ziqi Wu

Endokrynologia Polska 75:501-509 PubMed39376176

2024

The functional DIAPH3-FOXM1 interaction modulates the aggressive transformation of anaplastic thyroid carcinoma cells and Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Shi,Hao Fang,Kai Fu,Zhen Zhao,Fei Yang,Yan Liu

Translational cancer research 13:2437-2450 PubMed38881929

2024

Up-regulation of NCAPG mediated by E2F1 facilitates the progression of osteosarcoma through the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Luo,Haiyang Cheng,Jigeng Fan,Tianwei Sun

Diabetic medicine : a journal of the British Diabetic Association 40:e15051 PubMed36692102

2023

Identification of signalling downstream of the transcription factor forkhead box protein M1 that protects against endoplasmic reticulum stress in a diabetic foot ulcer model.

Applications

Unspecified application

Species

Unspecified reactive species

Weixia Peng,Ting Zhu,Guangda Xiang,Ting Ding,Jun Zhao,Dan Xiong,Yaqin Zhong,Youqi Zhang

Translational andrology and urology 11:1116-1129 PubMed36092834

2022

PRRX1/FOXM1 reduces gemcitabin-induced cytotoxicity by regulating autophagy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xixi Huang,Weiping Huang,Keming Wu,Qi Lin,Gang Chen

Frontiers in oncology 12:756109 PubMed35651785

2022

Cancer-Secreted Exosomal MiR-620 Inhibits ESCC Aerobic Glycolysis FOXM1/HER2 Pathway and Promotes Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbo Zhu,Fang Li,Yilong Wan,Hansi Liang,Si Li,Bo Peng,Liqun Shao,Yunyun Xu,Dong Jiang

Translational cancer research 11:414-425 PubMed35281418

2022

Knockdown of inhibits the progression of ovarian cancer and is related to the signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fanling Meng,Jing Ding,Wei Xu,Chang Luo,Xihai Chen,Ruichun Zhang,Lin Sui,Yuanlong Hu,Shuang Liu,Guangyue Shi,Yunlong He,Xin Ning,Rong Ma,Ning Huang

Cancer biotherapy & radiopharmaceuticals 34:572-580 PubMed31644316

2019

// Feedback Loop Regulates Papillary Thyroid Carcinoma Cell Proliferation and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Wang,Xin Wang,Ying Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXM1 antibody

Immunoprecipitation - Anti-FOXM1 antibody (AB245309)

Western blot - Anti-FOXM1 antibody (AB245309)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (10)

Product promise