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AB26069

Anti-FOXN1 antibody

3

(2 Reviews)

|

(2 Publications)

Goat Polyclonal FOXN1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FOXN1 aa 600 to C-terminus.

View Alternative Names

RONU, WHN, FOXN1, Forkhead box protein N1, Winged-helix transcription factor nude

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXN1 antibody (AB26069)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXN1 antibody (AB26069)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human thymus tissue labeling FOXN1 with ab26069 at 4 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXN1 antibody (AB26069)
  • IHC-P

AbReview50297****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXN1 antibody (AB26069)

Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human skin tissue sections, labelling FOXN1 with ab26069 at a dilution of 1/25 incubated for 18 hours at 4°C. Permeablization was with 0.05% Tween. Blocking was with 5% serum incubated for 20 minutes at room temperature. The secondary used was a swine polyclonal biotin-conjugate at 1/1000.

Image is courtesy of an AbReview submitted by Muriel Cario-Andre.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FOXN1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15353

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXN1 also known as Forkhead Box N1 is a transcription factor with an approximate mass of 48 kDa. It plays a role in regulating the expression of genes important for the development of certain tissues. FOXN1 is expressed predominantly in the thymus where it guides the differentiation of epithelial cells. Its expression is also observed in skin tissue implicating it in keratinocyte regulation.
Biological function summary

FOXN1 influences the differentiation and function of thymic epithelial cells which are essential for T-cell maturation. It does not associate with any specific protein complex but acts independently to modulate gene expression. By influencing these processes FOXN1 directly impacts immune function and epidermal integrity highlighting its significance in both immunity and skin health.

Pathways

FOXN1 is an important regulator within the T-cell development pathway impacting the maturation and selection of thymocytes. It aligns closely with the Wnt signaling pathway essential for cell proliferation and differentiation. Within these pathways FOXN1 interacts with other proteins like β-catenin an important mediator in Wnt signaling playing a role in cell fate determination.

Mutations or dysregulation of FOXN1 connect to immunodeficiencies and skin disorders. Nude/SCID phenotypes characterized by a lack of thymus and hair directly link to FOXN1 deficiency. Moreover alopecia a condition causing hair loss involves disruptions in FOXN1 signaling. Connections to other proteins such as CCL25 which impacts thymic development illustrate the broader implications of FOXN1 dysregulation in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed : 21507891).
See full target information FOXN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental animals 63:215-25 PubMed24770647

2014

Transcriptional and histological analyses of the thymic developmental process in the fetal pig.

Applications

IHC

Species

Pig

Shunichi Suzuki,Misae Suzuki,Michiko Nakai,Shoichiro Sembon,Daiichiro Fuchimoto,Akira Onishi

American journal of clinical pathology 138:103-14 PubMed22706865

2012

Thymic carcinoma, part 1: a clinicopathologic and immunohistochemical study of 65 cases.

Applications

IHC-P

Species

Human

Annikka Weissferdt,Cesar A Moran
View all publications
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Product promise

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