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AB96374

Anti-FOXN1 antibody

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(1 Publication)

Rabbit Polyclonal FOXN1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOXN1 aa 550 to C-terminus.
3 Images
Western blot - Anti-FOXN1 antibody (AB96374)
  • WB

Supplier Data

Western blot - Anti-FOXN1 antibody (AB96374)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FOXN1 antibody (ab96374) at 1/500 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Lane 3:

HCT116 whole cell extracts at 30 µg

Predicted band size: 69 kDa

false

Western blot - Anti-FOXN1 antibody (AB96374)
  • WB

Supplier Data

Western blot - Anti-FOXN1 antibody (AB96374)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FOXN1 antibody (ab96374) at 1/500 dilution

All lanes:

HeLa Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 69 kDa

false

Western blot - Anti-FOXN1 antibody (AB96374)
  • WB

Supplier Data

Western blot - Anti-FOXN1 antibody (AB96374)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FOXN1 antibody (ab96374) at 1/500 dilution

Lane 1:

Mouse thymus tissue extracts at 50 µg

Lane 2:

Rat thymus tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FOXN1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15353

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXN1 also known as Forkhead Box N1 is a transcription factor with an approximate mass of 48 kDa. It plays a role in regulating the expression of genes important for the development of certain tissues. FOXN1 is expressed predominantly in the thymus where it guides the differentiation of epithelial cells. Its expression is also observed in skin tissue implicating it in keratinocyte regulation.
Biological function summary

FOXN1 influences the differentiation and function of thymic epithelial cells which are essential for T-cell maturation. It does not associate with any specific protein complex but acts independently to modulate gene expression. By influencing these processes FOXN1 directly impacts immune function and epidermal integrity highlighting its significance in both immunity and skin health.

Pathways

FOXN1 is an important regulator within the T-cell development pathway impacting the maturation and selection of thymocytes. It aligns closely with the Wnt signaling pathway essential for cell proliferation and differentiation. Within these pathways FOXN1 interacts with other proteins like β-catenin an important mediator in Wnt signaling playing a role in cell fate determination.

Mutations or dysregulation of FOXN1 connect to immunodeficiencies and skin disorders. Nude/SCID phenotypes characterized by a lack of thymus and hair directly link to FOXN1 deficiency. Moreover alopecia a condition causing hair loss involves disruptions in FOXN1 signaling. Connections to other proteins such as CCL25 which impacts thymic development illustrate the broader implications of FOXN1 dysregulation in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed : 21507891).
See full target information FOXN1

Alternative Names

RONU, WHN, FOXN1, Forkhead box protein N1, Winged-helix transcription factor nude
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry :101775 PubMed35257748

2022

Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Guo,Hongjian Zhao,Haozhe Cheng,Honglei Kang,Yimin Dong,Renpeng Peng,Meipeng Zhu,Zhong Fang,Feng Li
View all publications
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Product promise

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