Rabbit Polyclonal FOXN1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOXN1 aa 550 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human FOXN1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15353
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Target data
Function
Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed:21507891).
Alternative names
Forkhead box protein N1, Winged-helix transcription factor nude, RONU, FOXN1, WHN
Recommended products
Rabbit Polyclonal FOXN1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOXN1 aa 550 to C-terminus.
Alternative names
Forkhead box protein N1, Winged-helix transcription factor nude, RONU, FOXN1, WHN
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human FOXN1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15353
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXN1 also known as Forkhead Box N1 is a transcription factor with an approximate mass of 48 kDa. It plays a role in regulating the expression of genes important for the development of certain tissues. FOXN1 is expressed predominantly in the thymus where it guides the differentiation of epithelial cells. Its expression is also observed in skin tissue implicating it in keratinocyte regulation.
- Biological function summary
FOXN1 influences the differentiation and function of thymic epithelial cells which are essential for T-cell maturation. It does not associate with any specific protein complex but acts independently to modulate gene expression. By influencing these processes FOXN1 directly impacts immune function and epidermal integrity highlighting its significance in both immunity and skin health.
- Pathways
FOXN1 is an important regulator within the T-cell development pathway impacting the maturation and selection of thymocytes. It aligns closely with the Wnt signaling pathway essential for cell proliferation and differentiation. Within these pathways FOXN1 interacts with other proteins like β-catenin an important mediator in Wnt signaling playing a role in cell fate determination.
- Associated diseases and disorders
Mutations or dysregulation of FOXN1 connect to immunodeficiencies and skin disorders. Nude/SCID phenotypes characterized by a lack of thymus and hair directly link to FOXN1 deficiency. Moreover alopecia a condition causing hair loss involves disruptions in FOXN1 signaling. Connections to other proteins such as CCL25 which impacts thymic development illustrate the broader implications of FOXN1 dysregulation in these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-FOXN1 antibody (ab96374)
7.5% SDS PAGE
All lanes: Western blot - Anti-FOXN1 antibody (ab96374) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 69 kDa
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