AB129453

Anti-FOXN3 antibody

Name: Anti-FOXN3 antibody (ab129453) | Abcam
Brand: Abcam Limited
SKU: AB129453
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal FOXN3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human FOXN3 aa 400-500.

View Alternative Names

C14orf116, CHES1, FOXN3, Forkhead box protein N3, Checkpoint suppressor 1

2 Images

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Immunoprecipitation - Anti-FOXN3 antibody (AB129453)

Detection of FOXN3 by Western Blot of Immunprecipitate.
Lane 1 : ab129453 at 1μg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FOXN3 antibody (ab129453)

Predicted band size: 54 kDa

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Western blot - Anti-FOXN3 antibody (AB129453)

All lanes:

Western blot - Anti-FOXN3 antibody (ab129453) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 54 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FOXN3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

O00409

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXN3 also known as Forkhead Box N3 is a transcription factor that regulates transcription of target genes. The protein belongs to the family of forkhead box transcription factors and possesses a conserved DNA-binding domain. FOXN3 has an approximate molecular weight of 74 kDa. It is expressed in various tissues including the liver heart and kidneys. This protein contributes significantly to cellular stress responses and developmental processes.

Biological function summary

FOXN3 modulates gene expression related to cell cycle control and apoptosis. It acts within a complex to influence the stability and transcriptional activity of other proteins. As part of the MLL complex FOXN3 co-regulates gene expression essential for hematopoietic stem cell maintenance. Through controlling specific target genes it plays a critical role in development and differentiation impacting both normal physiological processes and pathological conditions.

Pathways

FOXN3 is involved in the regulation of the p53 pathway a critical pathway for tumor suppression and cell cycle control. The protein collaborates with p53 to manage stress-induced transcriptional responses. In the NF-kB signaling pathway FOXN3 regulates immune and inflammatory responses positioning itself as an important mediator of immune functions. It interplays with proteins like MLL and p21 emphasizing its significance in these pathways.

FOXN3 associates with cancer particularly breast cancer contributing to its development and progression through aberrant regulation of cell cycle genes. Dysregulation of FOXN3 links to developmental disorders impacting genes that are essential during embryogenesis. In cancer FOXN3 interacts with proteins like BRCA1 amplifying the effects of its dysfunction in tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).

See full target information FOXN3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1865 PubMed39984467

2025

Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjin Yu,Yingke Li,Yiming Li,Xiaotian Liu,Qingyang Huo,Nan Wu,Yangxia Zhang,Taoling Zeng,Yong Zhang,Henry You Li,Jie Lian,Jihong Zhou,Emmanuel Jairaj Moses,Jian Geng,Juntang Lin,Wei Li,Xinxing Zhu

Cancer biology & therapy 25:2373497 PubMed38967961

2024

MiR-135b-5p promotes cetuximab resistance in colorectal cancer by regulating FOXN3.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Peng,Xiaoqing Li,Yuhui Yao,Yu Nie,Lingyao Fan,Chuandong Zhu

Clinical epigenetics 15:44 PubMed36945044

2023

DNA methylation abnormalities induced by advanced maternal age in villi prime a high-risk state for spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Qin,Wei Chen,Lingyue Hua,Yan Meng,Jing Wang,Hanna Li,Rui Yang,Liying Yan,Jie Qiao

Nucleic acids research 51:2195-2214 PubMed36794705

2023

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxing Zhu,Beijia Huang,Fengting Zhao,Jie Lian,Lixiang He,Yangxia Zhang,Longkai Ji,Jinghang Zhang,Xin Yan,Taoling Zeng,Chunya Ma,Yinming Liang,Chen Zhang,Juntang Lin

Cell death discovery 8:474 PubMed36450706

2022

Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Xu,Shuyan Liu,Chun Feng,Fuyi Xu,Demin Kong,Jia Mi,Chunhua Yang,Guilong Zhang,Pengfei Wei,Buyan-Ochir Orgil,Jonas Bergquist,Geng Tian

Oncology letters 18:1521-1529 PubMed31423219

2019

Novel tumor-suppressor FOXN3 is downregulated in adult acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hang He,Jinjing Zhang,Yi Qu,Yue Wang,Yan Zhang,Xiaojing Yan,Yan Li,Rui Zhang

Cell biology international 43:1113-1124 PubMed29972255

2018

MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Sun,Xiaona Ma,Chen Tu,Xiaopeng Wang,Jianqiang Qu,Shuang Wang,Shengxiang Xiao

Cell death & disease 9:559 PubMed29752474

2018

Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Xu,Yangyang Yang,Bowen Li,Yanan Li,Kangkai Xia,Yuxi Yang,Xiahui Li,Miao Wang,Shujing Li,Huijian Wu

The Journal of clinical investigation 127:3421-3440 PubMed28805661

2017

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjin Li,Zihan Zhang,Xinhua Liu,Xiao Cheng,Yi Zhang,Xiao Han,Yu Zhang,Shumeng Liu,Jianguo Yang,Bosen Xu,Lin He,Luyang Sun,Jing Liang,Yongfeng Shang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXN3 antibody

Immunoprecipitation - Anti-FOXN3 antibody (AB129453)

Western blot - Anti-FOXN3 antibody (AB129453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise