Anti-FOXN3 antibody [RP23040211]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXN3 antibody [RP23040211] (AB313485)

Immunofluorescent analysis of Hep G2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous FOXN3 with ab313485 at 2µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of FOXN3 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of FOXN3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-FOXN3 Antibody [RP23040211] (AB313485)

All lanes:

Western blot - Anti-FOXN3 Antibody [RP23040211] (ab313485) at 1 µg/mL

Lane 1:

Jurkat cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Lane 3:

KARPAS 299 cell lysate at 30 µg

Lane 4:

HT29 cell lysate at 30 µg

Lane 5:

THP1 cell lysate at 30 µg

Lane 6:

U937 cell lysate at 30 µg

Lane 7:

U2OS cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 54 kDa

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