AB179450

Anti-FOXO1A antibody [EPR12020]

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

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(51 Publications)

Anti-FOXO1A antibody [EPR12020] (ab179450) is a rabbit monoclonal antibody detecting FOXO1A in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

FKHR, FOXO1A, FOXO1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling FOXO1A with unpurified ab179450 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXO1A with unpurified ab179450 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling FOXO1A with purified ab179450 at 1/250 dilution (4.73 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

The molecular weight observed is consistent with what has been described in PMID : 20406953

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] (ab179450) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Lane 4:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 70-75 kDa

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Lab

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FOXO1A knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab179450 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.

Unpurified ab179450 was shown to specifically react with FOXO1 (FOXO1A) in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when FOXO1 (FOXO1A) knockout cells were examined. Wild-type and FOXO1 (FOXO1A) knockout samples were subjected to SDS-PAGE. ab179450 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] (ab179450)

Predicted band size: 69 kDa

false

Supplier Data

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-FOXO1A antibody (ab179450) staining at 1/1000 dilution. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 3 minutes.

All lanes:

Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (<a href='/en-us/products/primary-antibodies/foxo1a-pt24foxo3a-pt32foxo4-pt28foxo6-pt26-antibody-epr28359-78-ab312326'>ab312326</a>) at 1/1000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) transfected with mouse FOXO1A (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with mouse FOXO1A (WT) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg

Lane 3:

Untreated 293T transfected with mouse FOXO1A (Mutant T24A) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with mouse FOXO1A (Mutant T24A) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 78-82 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] (ab179450) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 69 kDa

false

Carrier free

Anti-FOXO1A antibody [EPR12020] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12020

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.Not suitable for Leica Bond system.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-FOXO1A antibody [EPR12020] (ab179450) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of FOXO1A?
Anti-FOXO1A [EPR12020] (ab179450) specifically detects a band for FOXO1A (UniProt: Q12778) at a molecular weight of 69kDa.

Trusted by the scientific community
Anti-FOXO1A [EPR12020] (ab179450) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-FOXO1A antibody [EPR12020] (ab179450) has been confirmed by Western blot testing in FOXO1 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR12020] also available for your convenience: ab179450, Carrier free - ab240212

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO1A also known as forkhead box O1 FOXO1 or FOXO1 transcription factor is a protein with a molecular weight of approximately 70 kDa. It is an important transcription factor in the FOXO family responsible for regulating gene expression in response to environmental and physiological stimuli. FOXO1A predominantly localizes in the nucleus but can translocate to the cytoplasm under certain conditions. Expression of FOXO1A occurs in various tissues including liver adipose tissue and pancreatic islets indicating its broad functional roles in different organs.

Biological function summary

FOXO1A acts as a regulator of metabolic pathways apoptosis and cell cycle regulation. Its role in glucose metabolism stands out as a critical function where it influences insulin signaling and glucose homeostasis. FOXO1A can also form complexes with other transcription factors like PPARγ enhancing its regulatory capacity. Interactions within these complexes dictate the transcriptional outcomes influencing cellular responses to stress and growth signals.

Pathways

The FOXO1 transcription factor plays a critical role in the insulin signaling and PI3K-Akt pathway. These pathways are essential for maintaining energy balance and cellular survival. FOXO1A interacts with proteins like Akt which phosphorylates FOXO1A resulting in its cytoplasmic sequestration and subsequent inactivation. This regulation is vital for insulin-mediated glucose uptake and overall metabolic homeostasis preserving cellular functions under fluctuating nutrient conditions.

Aberrant FOXO1A activity is associated with type 2 diabetes and certain cancers. Dysregulation leads to impaired insulin signaling and glucose metabolism contributing to insulin resistance and hyperglycemia in diabetes. In cancer altered FOXO1A expression affects apoptosis and cell proliferation influencing tumor progression and survival. Associations with proteins such as Akt and PPARγ in these conditions further exemplify its network in disease mechanisms highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed : 10358076, PubMed : 12228231, PubMed : 15220471, PubMed : 15890677, PubMed : 18356527, PubMed : 19221179, PubMed : 20543840, PubMed : 21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed : 10358076). Activity suppressed by insulin (PubMed : 10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability : when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1 (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1 (PubMed : 17024043). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed : 18356527, PubMed : 19221179). Promotes neural cell death (PubMed : 18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed : 20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Positive regulator of apoptosis in cardiac smooth muscle cells as a result of its transcriptional activation of pro-apoptotic genes (PubMed : 19483080). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed : 31063815).

See full target information FOXO1

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Current medical science 45:1244-1253 PubMed41060356

2025

miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Teng,Miao Tian,Xiao-Kang Luo,Qiu-Ping Jiang,Hai-Yun Yuan,Jian Zhuang,Ji-Mei Chen,Cheng-Bin Zhou

Histology and histopathology :18995 PubMed41017373

2025

UCHL1 enhances transcription by stabilizing FOXO1 through deubiquitination in knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jiawei Lu,Chonghao Gu,Zikang Xie,Zhongyu Xia,Bingqing Guo,Tao Jiang

Communications biology 8:1097 PubMed40702210

2025

Cathepsin B deficiency disrupts cortical development via PEG3, leading to depression-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Xie,Qinghu Yang,Fei Lan,Wei Kong,Shuxuan Zhao,Jinyi Sun,Yan Yan,Zhenzhen Quan,Zhantao Bai,Hong Qing,Jian Mao,Junjun Ni

The Tohoku journal of experimental medicine : PubMed40670092

2025

FOXO1 Targets LHX8 1 to Promote Granulosa Cell Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Luo,Man Luo,Licen Xie,Mi Tang,Minhui Luo,Jialan Cui

Biochemical genetics : PubMed40392448

2025

A Novel Telomere Maintenance Gene-Related Model for Prognosis Prediction in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Liang Wang,Xiao-Xia Xi,Jian-Hao Zheng

Cell death discovery 11:210 PubMed40301310

2025

Acetylated KIAA1429 by TIP60 facilitates metastasis and immune evasion of hepatocellular carcinoma via N6-methyladenosine-KDM5B-mediated regulation of FoxO1.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Quan,Huijun Zhou,Fei Chen,Jie Chen,Yun He,Hua Xiao,Jia Liu,Lei Shi,Wei Xie,Pan Chen,Jia Luo

BMC cancer 25:747 PubMed40264026

2025

FOXO1 mediates miR-99a-5p/E2F7 to restrain breast cancer cell proliferation and induce apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Haili Wang,Yiliang Wang,Bo Ma

BMC gastroenterology 25:283 PubMed40263992

2025

Remimazolam alleviates hepatic ischemia-reperfusion injury by activating FOXO1/3 signaling : Remimazolam alleviates hepatic ischemia reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhou,Jian Liu,Lu Jin,Xiaoling Huang

Brain and behavior 14:e70095 PubMed39682063

2024

Baihui-Penetrating-Qubin Acupuncture Attenuates Neurological Deficits Through SIRT1/FOXO1 Reducing Oxidative Stress and Neuronal Apoptosis in Intracerebral Hemorrhage Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Shan Dong,Ming-Yue Li,Xue-Ping Yu,Yu-Na Kan,Xiao-Hong Dai,Lei Zheng,Hong-Tao Cao,Wen-Hui Duan,En-Li Luo,Wei Zou

Nephron 149:226-239 PubMed39602888

2024

Diosgenin Improves Lipid Metabolism in Diabetic Nephropathy via Regulation of miR-148b-3p/DNMT1/FOXO1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Luo,Zongren Hu,Jichang Yang,Jinhan Yang,Wen Sheng,Chengxiong Lin,Dian Li,Qinghu He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXO1A antibody [EPR12020]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Western blot - Anti-FOXO1A antibody [EPR12020] (AB179450)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (51)

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