AB240212

Anti-FOXO1A antibody [EPR12020] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal FOXO1A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

FKHR, FOXO1A, FOXO1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXO1A with ab179450 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179450).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling FOXO1A with ab179450 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179450).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling FOXO1A with purified ab179450 at 1/250 dilution (4.73 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179450)

Lab

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

This data was developed using ab179450, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in PMID : 20406953

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Lane 4:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 70-75 kDa

false

Supplier Data

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

This data was developed using ab179450, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 69 kDa

false

Lab

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

This data was developed using ab179450, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FOXO1A knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab179450 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.

Unpurified ab179450 was shown to specifically react with FOXO1 (FOXO1A) in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when FOXO1 (FOXO1A) knockout cells were examined. Wild-type and FOXO1 (FOXO1A) knockout samples were subjected to SDS-PAGE. ab179450 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)

Predicted band size: 69 kDa

false

Unconjugated

Anti-FOXO1A antibody [EPR12020]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12020

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Not suitable for Leica Bond system.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab240212 is the carrier-free version of ab179450.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO1A also known as forkhead box O1 FOXO1 or FOXO1 transcription factor is a protein with a molecular weight of approximately 70 kDa. It is an important transcription factor in the FOXO family responsible for regulating gene expression in response to environmental and physiological stimuli. FOXO1A predominantly localizes in the nucleus but can translocate to the cytoplasm under certain conditions. Expression of FOXO1A occurs in various tissues including liver adipose tissue and pancreatic islets indicating its broad functional roles in different organs.

Biological function summary

FOXO1A acts as a regulator of metabolic pathways apoptosis and cell cycle regulation. Its role in glucose metabolism stands out as a critical function where it influences insulin signaling and glucose homeostasis. FOXO1A can also form complexes with other transcription factors like PPARγ enhancing its regulatory capacity. Interactions within these complexes dictate the transcriptional outcomes influencing cellular responses to stress and growth signals.

Pathways

The FOXO1 transcription factor plays a critical role in the insulin signaling and PI3K-Akt pathway. These pathways are essential for maintaining energy balance and cellular survival. FOXO1A interacts with proteins like Akt which phosphorylates FOXO1A resulting in its cytoplasmic sequestration and subsequent inactivation. This regulation is vital for insulin-mediated glucose uptake and overall metabolic homeostasis preserving cellular functions under fluctuating nutrient conditions.

Aberrant FOXO1A activity is associated with type 2 diabetes and certain cancers. Dysregulation leads to impaired insulin signaling and glucose metabolism contributing to insulin resistance and hyperglycemia in diabetes. In cancer altered FOXO1A expression affects apoptosis and cell proliferation influencing tumor progression and survival. Associations with proteins such as Akt and PPARγ in these conditions further exemplify its network in disease mechanisms highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed : 10358076, PubMed : 12228231, PubMed : 15220471, PubMed : 15890677, PubMed : 18356527, PubMed : 19221179, PubMed : 20543840, PubMed : 21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed : 10358076). Activity suppressed by insulin (PubMed : 10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability : when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1 (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1 (PubMed : 17024043). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed : 18356527, PubMed : 19221179). Promotes neural cell death (PubMed : 18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed : 20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Positive regulator of apoptosis in cardiac smooth muscle cells as a result of its transcriptional activation of pro-apoptotic genes (PubMed : 19483080). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed : 31063815).

See full target information FOXO1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biologics : targets & therapy 18:181-193 PubMed38979130

2024

STAT5B Suppresses Ferroptosis by Promoting DCAF13 Transcription to Regulate p53/xCT Pathway to Promote Mantle Cell Lymphoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Jun Zhang,Chong Ling Hu,Bing Ling Guo,Xi Ping Liang,Chao Yu Wang,Tao Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXO1A antibody [EPR12020] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise