Rabbit Recombinant Monoclonal FOXO1A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img171964.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)")
![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img185616.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61766.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)")
![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img183266.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74497.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (AB240212)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed:10358076). Activity suppressed by insulin (PubMed:10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1 (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1 (PubMed:17024043). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed:18356527, PubMed:19221179). Promotes neural cell death (PubMed:18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed:20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Positive regulator of apoptosis in cardiac smooth muscle cells as a result of its transcriptional activation of pro-apoptotic genes (PubMed:19483080). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815).
Alternative names
FKHR, FOXO1A, FOXO1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
Recommended products
Rabbit Recombinant Monoclonal FOXO1A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12020
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240212 is the carrier-free version of Anti-FOXO1A antibody [EPR12020] ab179450.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXO1A also known as forkhead box O1 FOXO1 or FOXO1 transcription factor is a protein with a molecular weight of approximately 70 kDa. It is an important transcription factor in the FOXO family responsible for regulating gene expression in response to environmental and physiological stimuli. FOXO1A predominantly localizes in the nucleus but can translocate to the cytoplasm under certain conditions. Expression of FOXO1A occurs in various tissues including liver adipose tissue and pancreatic islets indicating its broad functional roles in different organs.
- Biological function summary
FOXO1A acts as a regulator of metabolic pathways apoptosis and cell cycle regulation. Its role in glucose metabolism stands out as a critical function where it influences insulin signaling and glucose homeostasis. FOXO1A can also form complexes with other transcription factors like PPARγ enhancing its regulatory capacity. Interactions within these complexes dictate the transcriptional outcomes influencing cellular responses to stress and growth signals.
- Pathways
The FOXO1 transcription factor plays a critical role in the insulin signaling and PI3K-Akt pathway. These pathways are essential for maintaining energy balance and cellular survival. FOXO1A interacts with proteins like Akt which phosphorylates FOXO1A resulting in its cytoplasmic sequestration and subsequent inactivation. This regulation is vital for insulin-mediated glucose uptake and overall metabolic homeostasis preserving cellular functions under fluctuating nutrient conditions.
- Associated diseases and disorders
Aberrant FOXO1A activity is associated with type 2 diabetes and certain cancers. Dysregulation leads to impaired insulin signaling and glucose metabolism contributing to insulin resistance and hyperglycemia in diabetes. In cancer altered FOXO1A expression affects apoptosis and cell proliferation influencing tumor progression and survival. Associations with proteins such as Akt and PPARγ in these conditions further exemplify its network in disease mechanisms highlighting its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img171964.jpg "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
This data was developed using Anti-FOXO1A antibody [EPR12020] ab179450, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Predicted band size: 69 kDa
![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img185616.jpg "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
This data was developed using Anti-FOXO1A antibody [EPR12020] ab179450, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in PMID: 20406953
All lanes: Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg
Lane 2: Mouse heart lysates at 20 µg
Lane 3: Rat heart lysates at 20 µg
Lane 4: Rat spleen lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 70-75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61766.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling FOXO1A with purified Anti-FOXO1A antibody [EPR12020] ab179450 at 1/250 dilution (4.73 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXO1A antibody [EPR12020] ab179450)![Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--western-blot-img183266.jpg "Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
This data was developed using Anti-FOXO1A antibody [EPR12020] ab179450, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: FOXO1A knockout HAP1 whole cell lysate (20 μg)Lanes 1 - 2: Merged signal (red and green). Green - Anti-FOXO1A antibody [EPR12020] ab179450 observed at 69 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Unpurified Anti-FOXO1A antibody [EPR12020] ab179450 was shown to specifically react with FOXO1 (FOXO1A) in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when FOXO1 (FOXO1A) knockout cells were examined. Wild-type and FOXO1 (FOXO1A) knockout samples were subjected to SDS-PAGE. Anti-FOXO1A antibody [EPR12020] ab179450 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
Predicted band size: 69 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74497.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling FOXO1A with Anti-FOXO1A antibody [EPR12020] ab179450 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXO1A antibody [EPR12020] ab179450).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119251.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXO1A with Anti-FOXO1A antibody [EPR12020] ab179450 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXO1A antibody [EPR12020] ab179450).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212), expandable thumbnail](https://content.abcam.com/products/images/foxo1a-antibody-epr12020-bsa-and-azide-free-ab240212--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119251.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] - BSA and Azide free (ab240212)")
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