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AB283583

Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free

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Rabbit Recombinant Monoclonal FOXO1A phospho S253 antibody. Carrier free. Suitable for Dot, WB and reacts with Mouse, Rat samples.

View Alternative Names

Fkhr, Foxo1a, Foxo1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

3 Images
Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)
  • WB

Lab

Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)

This data was developed using ab259337, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] (<a href='/en-us/products/primary-antibodies/foxo1a-phospho-s253-antibody-epr24065-239-ab259337'>ab259337</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (Untreated membrane) at 20 µg

Lane 2:

PC-12 whole cell lysate (Phosphatase treated membrane) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 2:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)
  • WB

Lab

Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)

This data was developed using ab259337, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] (<a href='/en-us/products/primary-antibodies/foxo1a-phospho-s253-antibody-epr24065-239-ab259337'>ab259337</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

NIH/3T3 whole cell lysate (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

Dot Blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)
  • Dot

Supplier Data

Dot Blot - Anti-FOXO1A (phospho S253) antibody [EPR24065-239] - BSA and Azide free (AB283583)

This data was developed using ab259337, the same antibody clone in a different buffer formulation.

Dot blot analysis of FOXO1 (phospho Ser253) using ab259337 at 1/1000 (0.429 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : Mouse FOXO1 phospho-S253 peptide

Lane 2 : Unmodified mouse FOXO1 peptide

Lane 3 : Mouse FOXO3 phospho-S252 peptide

Lane 4 : Mouse FOXO4 phospho-S197 peptide

Lane 5 : Mouse FOXO6 phospho-S184 peptide

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

  • Unconjugated

    Anti-FOXO1A (phospho S253) antibody [EPR24065-239]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24065-239

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab283583 is the carrier-free version of ab259337.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO1A also known as forkhead box O1 FOXO1 or FOXO1 transcription factor is a protein with a molecular weight of approximately 70 kDa. It is an important transcription factor in the FOXO family responsible for regulating gene expression in response to environmental and physiological stimuli. FOXO1A predominantly localizes in the nucleus but can translocate to the cytoplasm under certain conditions. Expression of FOXO1A occurs in various tissues including liver adipose tissue and pancreatic islets indicating its broad functional roles in different organs.
Biological function summary

FOXO1A acts as a regulator of metabolic pathways apoptosis and cell cycle regulation. Its role in glucose metabolism stands out as a critical function where it influences insulin signaling and glucose homeostasis. FOXO1A can also form complexes with other transcription factors like PPARγ enhancing its regulatory capacity. Interactions within these complexes dictate the transcriptional outcomes influencing cellular responses to stress and growth signals.

Pathways

The FOXO1 transcription factor plays a critical role in the insulin signaling and PI3K-Akt pathway. These pathways are essential for maintaining energy balance and cellular survival. FOXO1A interacts with proteins like Akt which phosphorylates FOXO1A resulting in its cytoplasmic sequestration and subsequent inactivation. This regulation is vital for insulin-mediated glucose uptake and overall metabolic homeostasis preserving cellular functions under fluctuating nutrient conditions.

Aberrant FOXO1A activity is associated with type 2 diabetes and certain cancers. Dysregulation leads to impaired insulin signaling and glucose metabolism contributing to insulin resistance and hyperglycemia in diabetes. In cancer altered FOXO1A expression affects apoptosis and cell proliferation influencing tumor progression and survival. Associations with proteins such as Akt and PPARγ in these conditions further exemplify its network in disease mechanisms highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed : 12219087, PubMed : 12754525, PubMed : 15184386, PubMed : 15220471, PubMed : 16917544, PubMed : 17090532, PubMed : 17627282, PubMed : 17681146, PubMed : 20519497, PubMed : 20668652, PubMed : 21196578, PubMed : 21335550, PubMed : 21471200, PubMed : 22298775, PubMed : 22417654, PubMed : 22510882, PubMed : 27457971, PubMed : 34914893). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed : 17090532, PubMed : 21335550). Activity suppressed by insulin (PubMed : 12754525, PubMed : 17627282). Main regulator of redox balance and osteoblast numbers and controls bone mass (PubMed : 21471200, PubMed : 22298775). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (PubMed : 21471200, PubMed : 22298775). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability : when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (PubMed : 32103177). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (PubMed : 22298775). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (PubMed : 21471200). Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1 (PubMed : 12219087, PubMed : 27457971). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (PubMed : 12754525, PubMed : 25009184). Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1 (By similarity). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (By similarity). Promotes neural cell death (By similarity). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (PubMed : 21196578). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (By similarity). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Positive regulator of apoptosis in cardiac smooth muscle cells as a result of its transcriptional activation of pro-apoptotic genes (By similarity). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (By similarity).
See full target information Foxo1 phospho S253
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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