AB128876

Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal FOXO4/AFX phospho T32 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AFX, AFX1, MLLT7, FOXO4, Forkhead box protein O4, Fork head domain transcription factor AFX1

2 Images

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Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (AB128876)

All lanes:

Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (ab128876) at 1/1000 dilution

Lane 1:

293T cell lysates

Lane 2:

293T transfected with HA-FOXO4/AFX cell lysates

Lane 3:

293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates

Predicted band size: 53 kDa

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OI-RD Scanning - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (AB128876)

OI-RD Scanning

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OI-RD Scanning - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (AB128876)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2314(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO1A FOXO4/AFX FOXO3A and Foxo6 are proteins that belong to the Forkhead box O (FOXO) transcription factors family known for their role in regulating gene expression linked to various cellular processes. These proteins including FOXO3A with a molecular weight of approximately 71 kDa are primarily expressed in multiple tissues such as the brain liver and adipose tissue. These transcription factors act mechanically by binding to specific DNA sequences to control the expression of genes involved in metabolism cell cycle and apoptosis.

Biological function summary

FOXO proteins influence numerous cellular functions through their role in gene transcription. They are often considered part of larger complexes interacting with various cofactors and other proteins to modulate gene expression. FOXO3A for example plays a role in cellular antioxidant defense energy metabolism and maintenance of cellular homeostasis. Their activity can be modulated by post-translational modifications such as phosphorylation which influences their localization between the cytoplasm and nucleus affecting their function.

Pathways

FOXO proteins are integral components of the insulin-signaling pathway and the PI3K/Akt pathway. Through these pathways they interact closely with proteins such as Akt and PI3K which regulate their activity and subcellular localization. The insulin pathway modulates metabolic processes and glucose homeostasis while the PI3K/Akt pathway is involved in cell survival and growth. Their regulatory functions highlight their adaptive response to environmental and internal cellular signals.

FOXO proteins link to conditions like cancer and diabetes. In cancer FOXO3A is often implicated in tumor suppression where its inactivation can lead to an increase in cell proliferation and survival. The loss or mutation of FOXO function may contribute to tumorigenesis. In diabetes FOXO proteins relate to insulin regulation and resistance impacting metabolic syndrome and glucose intolerance. The interplay with proteins like Akt during these diseases can alter their transcriptional activity highlighting their significance in disease progression and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

See full target information FOXO4 phospho T32

Additional targets

FOXO3 phospho T32,FOXO6 phospho T26,FOXO1 phospho T24

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 12:713-728 PubMed35261797

2022

Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsuan Hung,Ming-Derg Lai,Wen-Chun Hung,Li-Tzong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody

Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (AB128876)

OI-RD Scanning - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (AB128876)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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