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Rabbit Polyclonal FOXO3A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 27 publications.


Images

Western blot - Anti-FOXO3A antibody (AB23683), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (AB23683), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (AB23683), expandable thumbnail
  • Western blot - Anti-FOXO3A antibody (AB23683), expandable thumbnail
  • Western blot - Anti-FOXO3A antibody (AB23683), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Dog
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info
5 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Dog

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1 µg/mL
Notes

-

Species

Rat

Dilution info
1 µg/mL
Notes

-

Species

Human

Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species

Dog

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882, PubMed:30513302). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed:30513302).

Alternative names

Recommended products

Rabbit Polyclonal FOXO3A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 27 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

Replenishment batches of our polyclonal antibody, ab23683 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab109629.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOXO3A also known as FOXO3 or 1e2 is a transcription factor with a molecular weight of approximately 71 kDa. It belongs to the Forkhead box O family of proteins. This protein plays an important role in the regulation of transcription in response to oxidative stress and growth stimuli. FOXO3A is expressed in a variety of tissues including liver muscle and brain where it contributes to a wide range of cellular processes related to stress resistance and metabolism. Scientists frequently study FOXO3A using techniques like FOXO3A ELISA and apply FOXO3A anticuerpos in research to understand its function more intricately.

Biological function summary

FOXO3A acts as a regulator of genes involved in cell cycle arrest apoptosis and DNA repair. It is not part of a simple protein complex but works closely with multiple factors to influence cellular homeostasis. Significantly FOXO3A controls the expression of Bcl-2 a protein important for cell survival and the protein p27 which is important for cell cycle control. FOXO3A's activity modulates cellular longevity and impacts stress responses highlighting its abundant biological functions.

Pathways

FOXO3A integrates into the PI3K/AKT and the insulin signaling pathways both essential for cellular metabolism and proliferation control. In the PI3K/AKT pathway FOXO3A functionally links with proteins such as AKT and PTEN where its activity can be inhibited by AKT-mediated phosphorylation. In doing so it supports a balance between cell survival and apoptosis allowing the cell to adjust to growth conditions and external stressors effectively.

Associated diseases and disorders

FOXO3A is linked to cancer and neurodegenerative diseases. In cancer alterations in FOXO3A activity or expression can affect tumor growth and resistance to oxidative stress often involving interactions with proteins like MDM2. Similarly in neurodegenerative disorders such as Alzheimer's disease FOXO3A modifies responses to oxidative damage potentially tied to proteins like Tau. Both instances highlight the role of FOXO3A as a versatile modulator that can influence the progression and management of various diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Western blot - Anti-FOXO3A antibody (ab23683)

    Lanes 1 - 4: Merged signal (red and green). Green - ab23683 observed at 82 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab23683 was shown to react with FOXO3A in wild-type cells in Western blot with loss of signal observed in FOXO3 knockout cell lines. Wild-type and FOXO3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab23683 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-FOXO3A antibody (ab23683) at 1 µg/mL

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human FOXO3 (FOXO3A) knockout HEK-293T cell lysate (Human FOXO3 (FOXO3A) knockout HEK-293T cell lysate ab256922) at 20 µg

    Lane 3: Wild-type HEK-293 cell lysate at 20 µg

    Lane 4: Western blot - Human FOXO3 (FOXO3A) knockout HEK-293 cell lysate (Human FOXO3 (FOXO3A) knockout HEK-293 cell lysate ab261649) at 20 µg

    Performed under reducing conditions.

    Predicted band size: 71 kDa

    Observed band size: 82 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (ab23683)

    ICC/IF image of ab23683 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% HepG2 fixed (10 min) HepG2 cells at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab23683)

    IHC image of FOXO3A staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23683, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Western blot - Anti-FOXO3A antibody (ab23683)

    Lanes 1 - 4: Merged signal (red and green). Green - ab23683 observed at 71 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab23683 was shown to recognize FOXO3A in wild-type HEK-293 cells as signal was lost at the expected MW in FOXO3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXO3 knockout samples were subjected to SDS-PAGE. ab23683 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-FOXO3A antibody (ab23683) at 1 µg/mL

    Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: FOXO3 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 71 kDa

    Observed band size: 82 kDa

  • Western blot - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Western blot - Anti-FOXO3A antibody (ab23683)

    ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727). It also detects a band at 50 kDa which could be a cleavage fragment. Both bands are partially blocked by the addition of the immunizing peptide.

    All lanes: Western blot - Anti-FOXO3A antibody (ab23683) at 1 µg/mL

    All lanes: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg

    Predicted band size: 71 kDa

    Observed band size: 50 kDa, 90 kDa

  • Western blot - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Western blot - Anti-FOXO3A antibody (ab23683)

    ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727).

    All lanes: Western blot - Anti-FOXO3A antibody (ab23683) at 1 µg/mL

    Lane 1: Heart (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 2: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 3: Heart (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 71 kDa

    Observed band size: 16 kDa, 27 kDa, 90 kDa

  • Western blot - Anti-FOXO3A antibody (ab23683), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-FOXO3A antibody (ab23683)

    FOXO3A western blot using anti-FOXO3A antibody ab23683. Publication image and figure legend from Gao, J., Liu, S., et al., 2018, Front Mol Neurosci, PubMed 30104959.

    ab23683 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab23683 please see the product overview.

    Mitochondrial respiratory chain and silent mating-type information regulation 2 homolog 3 (Sirt3) pathway mediated the protective effects of TLB in PC12 cells. Following pre-treatment with or without TLB for 1 h and incubated with 400 μM H2O2 for further 48 h, and the mitochondria in each group were isolated and purified. (A) Representative Western blots were shown for Sirt3, IDH2, forkheadboxO3a (FoxO3a)-, ac-SOD2, GPX-1 and uncoupling protein 2 (UCP2) proteins. (B) Quantitation of Sirt3 protein. (C) Quantitation of IDH2 protein. (D) Quantitation of Foxo3a protein. (E) Quantitation of ac-SOD2 protein. (F) Quantitation of GPX-1 protein. (G) Quantitation of UCP2 protein. (H) mRNA level of Sirt3 and mtDNA genes. (I) The activity of mitochondrial respiratory chain complex I. (J) The activity of adenosine triphosphate (ATP) (K) Nicotinamide adenine dinucleotide (NAD)+/NADH ratio. Data were presented as mean ± SD of three independent experiments. **P < 0.01 vs. untreated control cells; #P < 0.05, ##P < 0.01 vs. H2O2-treated cells.

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