Skip to main content

Rabbit Recombinant Monoclonal FOXO3A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXO3 aa 450-500.

Be the first to review this product! Submit a review

Images

Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (AB314087), expandable thumbnail
  • Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (AB314087), expandable thumbnail
  • Immunoprecipitation - Anti-FOXO3a antibody [BLR140H] - BSA free (AB314087), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human FOXO3 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link O43524

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882, PubMed:30513302). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed:30513302).

Alternative names

FKHRL1, FOXO3A, FOXO3, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

Recommended products

Rabbit Recombinant Monoclonal FOXO3A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXO3 aa 450-500.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human FOXO3 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link O43524
Clone number
BLR140H
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXO3A also known as FOXO3 or 1e2 is a transcription factor with a molecular weight of approximately 71 kDa. It belongs to the Forkhead box O family of proteins. This protein plays an important role in the regulation of transcription in response to oxidative stress and growth stimuli. FOXO3A is expressed in a variety of tissues including liver muscle and brain where it contributes to a wide range of cellular processes related to stress resistance and metabolism. Scientists frequently study FOXO3A using techniques like FOXO3A ELISA and apply FOXO3A anticuerpos in research to understand its function more intricately.

Biological function summary

FOXO3A acts as a regulator of genes involved in cell cycle arrest apoptosis and DNA repair. It is not part of a simple protein complex but works closely with multiple factors to influence cellular homeostasis. Significantly FOXO3A controls the expression of Bcl-2 a protein important for cell survival and the protein p27 which is important for cell cycle control. FOXO3A's activity modulates cellular longevity and impacts stress responses highlighting its abundant biological functions.

Pathways

FOXO3A integrates into the PI3K/AKT and the insulin signaling pathways both essential for cellular metabolism and proliferation control. In the PI3K/AKT pathway FOXO3A functionally links with proteins such as AKT and PTEN where its activity can be inhibited by AKT-mediated phosphorylation. In doing so it supports a balance between cell survival and apoptosis allowing the cell to adjust to growth conditions and external stressors effectively.

Associated diseases and disorders

FOXO3A is linked to cancer and neurodegenerative diseases. In cancer alterations in FOXO3A activity or expression can affect tumor growth and resistance to oxidative stress often involving interactions with proteins like MDM2. Similarly in neurodegenerative disorders such as Alzheimer's disease FOXO3A modifies responses to oxidative damage potentially tied to proteins like Tau. Both instances highlight the role of FOXO3A as a versatile modulator that can influence the progression and management of various diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087), expandable thumbnail

    Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087)

    Samples: Whole cell lysate (10 µg) from HEK293T, LNCaP, Hep-G2, RKO, Jurkat, MCF-7, GaMG, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody [BLR140H] (A700-140CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.

    All lanes: Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087) at 1/1000 dilution

    Lane 1: HEK293T Whole cell lysate at 10 µg

    Lane 2: LNCaP Whole cell lysate at 10 µg

    Lane 3: Hep-G2 Whole cell lysate at 10 µg

    Lane 4: RKO Whole cell lysate at 10 µg

    Lane 5: Jurkat Whole cell lysate at 10 µg

    Lane 6: MCF-7 Whole cell lysate at 10 µg

    Lane 7: GaMG Whole cell lysate at 10 µg

    Lane 8: HeLa Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087), expandable thumbnail

    Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087)

    Samples: Whole cell lysate (10 µg) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody [BLR140H] (A700-140CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.

    All lanes: Western blot - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087) at 1/1000 dilution

    Lane 1: BW5147.3 Whole cell lysate at 10 µg

    Lane 2: mIMCD-3 Whole cell lysate at 10 µg

    Lane 3: A20 Whole cell lysate at 10 µg

    Lane 4: NIH 3T3 Whole cell lysate at 10 µg

    Lane 5: CT26 Whole cell lysate at 10 µg

    Lane 6: CH27 Whole cell lysate at 10 µg

    Lane 7: C2C12 Whole cell lysate at 10 µg

    Lane 8: TCMK-1 Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087), expandable thumbnail

    Immunoprecipitation - Anti-FOXO3a antibody [BLR140H] - BSA free (ab314087)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXO3a recombinant monoclonal antibody [BLR140H] (A700-140) used for IP at 20 µl/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibodies A300-453A and A300-454A. For blotting immunoprecipitated FOXO3a, A700-140 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com