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AB232393

Anti-FOXO3A antibody [EPR1950] - BSA and Azide free

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Rabbit Recombinant Monoclonal FOXO3A antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

FKHRL1, FOXO3A, FOXO3, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

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Flow Cytometry (Intracellular) - Anti-FOXO3A antibody [EPR1950] - BSA and Azide free (AB232393)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FOXO3A antibody [EPR1950] - BSA and Azide free (AB232393)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO3A with unpurified ab109629 at 1/150 dilution (10μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109629).

Western blot - Anti-FOXO3A antibody [EPR1950] - BSA and Azide free (AB232393)
  • WB

Lab

Western blot - Anti-FOXO3A antibody [EPR1950] - BSA and Azide free (AB232393)

This data was developed using the same antibody clone in a different buffer formulation (ab109629).

Lanes 1 - 4 : Merged signal (red and green). Green - ab109629 observed at 82 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab109629 was shown to react with FOXO3A in wild-type cells in Western blot with loss of signal observed in FOXO3 knockout cellS. Wild-type and FOXO3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab109629 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-FOXO3A antibody [EPR1950] (<a href='/en-us/products/primary-antibodies/foxo3a-antibody-epr1950-ab109629'>ab109629</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human FOXO3 (FOXO3A) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-foxo3-foxo3a-knockout-hek-293t-cell-lysate-ab256922'>ab256922</a>) at 20 µg

Lane 2:

Western blot - Human FOXO3 (FOXO3A) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-foxo3-foxo3a-knockout-hek-293t-cell-line-ab265069'>ab265069</a>)

Lane 3:

Wild-type HEK-293 cell lysate at 20 µg

Lane 4:

Western blot - Human FOXO3 (FOXO3A) knockout HEK-293 cell lysate (<a href='/en-us/products/cell-lysates/human-foxo3-foxo3a-knockout-hek-293-cell-lysate-ab261649'>ab261649</a>) at 20 µg

Predicted band size: 71 kDa

Observed band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1950

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab232393 is the carrier-free version of ab109629.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO3A also known as FOXO3 or 1e2 is a transcription factor with a molecular weight of approximately 71 kDa. It belongs to the Forkhead box O family of proteins. This protein plays an important role in the regulation of transcription in response to oxidative stress and growth stimuli. FOXO3A is expressed in a variety of tissues including liver muscle and brain where it contributes to a wide range of cellular processes related to stress resistance and metabolism. Scientists frequently study FOXO3A using techniques like FOXO3A ELISA and apply FOXO3A anticuerpos in research to understand its function more intricately.
Biological function summary

FOXO3A acts as a regulator of genes involved in cell cycle arrest apoptosis and DNA repair. It is not part of a simple protein complex but works closely with multiple factors to influence cellular homeostasis. Significantly FOXO3A controls the expression of Bcl-2 a protein important for cell survival and the protein p27 which is important for cell cycle control. FOXO3A's activity modulates cellular longevity and impacts stress responses highlighting its abundant biological functions.

Pathways

FOXO3A integrates into the PI3K/AKT and the insulin signaling pathways both essential for cellular metabolism and proliferation control. In the PI3K/AKT pathway FOXO3A functionally links with proteins such as AKT and PTEN where its activity can be inhibited by AKT-mediated phosphorylation. In doing so it supports a balance between cell survival and apoptosis allowing the cell to adjust to growth conditions and external stressors effectively.

FOXO3A is linked to cancer and neurodegenerative diseases. In cancer alterations in FOXO3A activity or expression can affect tumor growth and resistance to oxidative stress often involving interactions with proteins like MDM2. Similarly in neurodegenerative disorders such as Alzheimer's disease FOXO3A modifies responses to oxidative damage potentially tied to proteins like Tau. Both instances highlight the role of FOXO3A as a versatile modulator that can influence the progression and management of various diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed : 10102273, PubMed : 16751106, PubMed : 21329882, PubMed : 30513302). Acts as a positive regulator of autophagy in skeletal muscle : in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed : 10102273, PubMed : 16751106). Participates in post-transcriptional regulation of MYC : following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed : 21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed : 23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability : when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed : 30513302).
See full target information FOXO3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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