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Rabbit Recombinant Monoclonal FOXO3A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-FOXO3A antibody [EPR29854-587] (AB323467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (AB323467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (AB323467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (AB323467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (AB323467), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882, PubMed:30513302). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed:30513302).

Additional Targets

Foxo3, FOXO3

Alternative names

FKHRL1, FOXO3A, FOXO3, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

Rabbit Recombinant Monoclonal FOXO3A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29854-587
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1, 3: 10 seconds, lanes 2, 4-6: 37 seconds

    All lanes: Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 6: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 82 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling FOXO3A with ab323467 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung cancer.

    The section was incubated with ab323467 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling FOXO3A with ab323467 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with ab323467 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of stomach tissue labeling FOXO3A with ab323467 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human adenocarcinoma of stomach (PMID: 33795838).

    The section was incubated with ab323467 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXO3A with ab323467 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon (PMID: 23828518).

    The section was incubated with ab323467 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 15 kDa (in lane 6) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 37 seconds, lanes 3-6: 180 seconds

    All lanes: Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse pancreas tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 82 kDa, 36 kDa

  • Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467), expandable thumbnail

    Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In lanes 1-3, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (82 kDa) are likely to be degradation products. In lane 4, To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    The identity of the higher MW band at approximately 150 kDa (in lanes 1-3) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 180 seconds, lane 4: 37 seconds

    All lanes: Western blot - Anti-FOXO3A antibody [EPR29854-587] (ab323467) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting FOXO3A whole cell lysate at 20 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 82 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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