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AB154786

Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

5

(2 Reviews)

|

(56 Publications)

Rabbit Recombinant Monoclonal FOXO3A phospho S253 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 56 publications.

View Alternative Names

FKHRL1, FOXO3A, FOXO3, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)

Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)
  • WB

Lab

Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786) at 1/5000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

MCF-7 (Human breast adenocarcinoma epithelial cell) treated with IGF-1. Whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 90 kDa

false

Exposure time: 1min

Dot Blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)
  • Dot

Lab

Dot Blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (AB154786)

Dot Blot analysis of Lane 1 : FOXO3A (pS253) phospho peptide and Lane 2 : FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 3 minutes.

  • Carrier free

    Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 660 APC

    APC Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 578 PE

    PE Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1951(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXO3A also known as FOXO3 or 1e2 is a transcription factor with a molecular weight of approximately 71 kDa. It belongs to the Forkhead box O family of proteins. This protein plays an important role in the regulation of transcription in response to oxidative stress and growth stimuli. FOXO3A is expressed in a variety of tissues including liver muscle and brain where it contributes to a wide range of cellular processes related to stress resistance and metabolism. Scientists frequently study FOXO3A using techniques like FOXO3A ELISA and apply FOXO3A anticuerpos in research to understand its function more intricately.
Biological function summary

FOXO3A acts as a regulator of genes involved in cell cycle arrest apoptosis and DNA repair. It is not part of a simple protein complex but works closely with multiple factors to influence cellular homeostasis. Significantly FOXO3A controls the expression of Bcl-2 a protein important for cell survival and the protein p27 which is important for cell cycle control. FOXO3A's activity modulates cellular longevity and impacts stress responses highlighting its abundant biological functions.

Pathways

FOXO3A integrates into the PI3K/AKT and the insulin signaling pathways both essential for cellular metabolism and proliferation control. In the PI3K/AKT pathway FOXO3A functionally links with proteins such as AKT and PTEN where its activity can be inhibited by AKT-mediated phosphorylation. In doing so it supports a balance between cell survival and apoptosis allowing the cell to adjust to growth conditions and external stressors effectively.

FOXO3A is linked to cancer and neurodegenerative diseases. In cancer alterations in FOXO3A activity or expression can affect tumor growth and resistance to oxidative stress often involving interactions with proteins like MDM2. Similarly in neurodegenerative disorders such as Alzheimer's disease FOXO3A modifies responses to oxidative damage potentially tied to proteins like Tau. Both instances highlight the role of FOXO3A as a versatile modulator that can influence the progression and management of various diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed : 10102273, PubMed : 16751106, PubMed : 21329882, PubMed : 30513302). Acts as a positive regulator of autophagy in skeletal muscle : in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed : 10102273, PubMed : 16751106). Participates in post-transcriptional regulation of MYC : following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed : 21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed : 23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability : when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed : 30513302).
See full target information FOXO3 phospho S253
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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 44:31-39 PubMed40493899

2025

lncRNA PTCSC1 Promotes TRAIL Resistance through FOXO3a Pathway in HCT116 and SW480 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Changcheng Wang,Jia Guo,Zengan Wu

Theranostics 15:3877-3899 PubMed40213661

2025

An anti-FAP-scFv-functionalized exosome-carrying hydrogel delivers mRNA to fibrotic nucleus pulposus cells to alleviate intervertebral disc degeneration by regulating FOXO3.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Li,Yu Zhai,Tianling Wang,Siya Wang,Yu Tian,Zhiqun Bian,Yang Zhang,Chao Zhang,Minghan Liu,Changqing Li

Science advances 11:eadr7208 PubMed40203118

2025

Silicate-based therapy for inflammatory dilated cardiomyopathy by inhibiting the vicious cycle of immune inflammation via FOXO signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Sun,Zhaowenbin Zhang,Fei Gao,Chen Yang,Ge Mang,Shuai Fu,Jiawei Tian,Jiang Chang

NPJ precision oncology 9:80 PubMed40119070

2025

ARRB2 promotes cervical cancer progression via stabilizing CDC25A mRNA through m6A-IGF2BP1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Li,Jie Zeng,Mengying Liu,Hui Cheng,Yuyin He,Sili He,Chen Wang

BJOG : an international journal of obstetrics and gynaecology 132:107-119 PubMed39973029

2025

Effects of Gonadotropin-Releasing Hormone Analogues on Ovarian Function and Embryogenesis: A Cyclophosphamide-Induced Mouse Model Study.

Applications

Unspecified application

Species

Unspecified reactive species

Qiwang Lin,Mingzhu Cao,Zijin Xu,He Fei,Yunfeng Jin,Jianqiao Liu,Hua Jiang

Biological & pharmaceutical bulletin 47:1774-1785 PubMed39477471

2024

5-Demethylnobiletin Ameliorates Isoproterenol-Induced Cardiac Fibrosis and Apoptosis by Repressing the Sirt1/FOXO3a/NF-κB and Wnt/β-Catenin Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Du,Weizhong Huangfu,Zhonghua Liu,Gaopeng Jia,Feng Zhao,Wenjun Cheng

Aging 16:685-700 PubMed38217540

2024

ZIP4 upregulation aggravates nucleus pulposus cell degradation by promoting inflammation and oxidative stress by mediating the HDAC4-FoxO3a axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingkui Shen,Kuankuan Li,Lulu Wang,Li Feng,Xinyu Zhang,Haoping Zhang,Honggang Zhou,Guoxian Pei

Nature communications 14:505 PubMed36720864

2023

Proteogenomics of diffuse gliomas reveal molecular subtypes associated with specific therapeutic targets and immune-evasion mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Wang,Rongkui Luo,Xuan Zhang,Hang Xiang,Bing Yang,Jinwen Feng,Mengjie Deng,Peng Ran,Akesu Sujie,Fan Zhang,Jiajun Zhu,Subei Tan,Tao Xie,Pin Chen,Zixiang Yu,Yan Li,Dongxian Jiang,Xiaobiao Zhang,Jian-Yuan Zhao,Yingyong Hou,Chen Ding

Developmental neuroscience 45:37-52 PubMed36470227

2022

miR-506-3p Relieves Neuropathic Pain following Brachial Plexus Avulsion via Mitigating Microglial Activation through Targeting the CCL2-CCR2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Jin,Wei Zheng,Songyuan Chi,Taihao Cui,Wei He

Nature cardiovascular research 1:1195-1214 PubMed39196168

2022

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Liyong Zhang,David Smyth,Mohammad Al-Khalaf,Alice Blet,Qiujiang Du,Jordan Bernick,Michael Gong,Xu Chi,Yena Oh,Malaika Roba-Oshin,Elizabeth Coletta,Michel Feletou,Anthony O Gramolini,Kyoung-Han Kim,Thais Coutinho,James L Januzzi,Benoit Tyl,Andre Ziegler,Peter P Liu
View all publications
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