Rabbit Recombinant Monoclonal FOXO4/AFX antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 21 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | WB | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Tested | Not recommended | Tested |
Mouse | Predicted | Predicted | Not recommended | Predicted |
Rat | Predicted | Predicted | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
AFX, AFX1, MLLT7, FOXO4, Forkhead box protein O4, Fork head domain transcription factor AFX1
Rabbit Recombinant Monoclonal FOXO4/AFX antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 21 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
This antibody was developed as part of a collaboration between the University of Utrecht in the Netherlands and the lab of Tobias Dansen.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
FOXO4 also known as AFX is part of the FoxO family of transcription factors and weighs about 74 kDa. It operates as an important player in transcriptional regulation by binding to specific DNA sequences. This protein modulates gene expression linked to apoptosis cell cycle regulation and oxidative stress resistance. FOXO4 commonly expresses in tissues like the liver skeletal muscle and adipose tissue where it carries out critical cellular functions.
This protein influences processes essential for cell survival and metabolism. FOXO4 forms part of the larger forkhead box family of transcription factors. Inside cells it regulates genes involved in mechanisms such as gluconeogenesis and immune responses. It can also interact with other proteins like 14-3-3 which helps control its nuclear-cytoplasmic shuttling impacting its activity.
FOXO4 becomes an integral part of the insulin signaling and oxidative stress response pathways. In the insulin pathway FOXO4 activity suppresses in response to insulin signals that trigger the PI3K/Akt pathway. Akt phosphorylates FOXO4 disallowing it from entering the nucleus. FOXO4 relates closely with FOXO1 and FOXO3 within these pathways where their coordinated activities help maintain energy homeostasis and cellular stress responses.
FOXO4 involvement becomes apparent in conditions such as diabetes and cancer. In diabetes disrupted insulin signaling impacts FOXO4 activity impairing glucose metabolism. In cancer FOXO4's role in cell cycle arrest and apoptosis associates it with tumor suppression and progression. Its interaction with proteins like p53 influences cellular responses to oncogenic stress marking FOXO4 as a significant factor in oncological research.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Purified ab128908 at 1/100 dilution (2μg) immunoprecipitating FOXO4/AFX in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab128908 + 293T whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab128908 in 293T whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 65 kDa
All lanes: Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
Predicted band size: 53 kDa
All lanes: Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution
Lane 1: 293T lysate at 10 µg
Lane 2: Fetal heart lysate at 10 µg
Lane 3: Fetal muscle lysate at 10 µg
Lane 4: Fetal lung lysate at 10 µg
Lane 5: HuT-78 lysate at 10 µg
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 65 kDa
Intracellular flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).
All lanes: Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution
Lane 1: 293T transfected with FOXO4/AFX
Lane 2: 293T cell lysate
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 63 kDa
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands above 100 kDa are unknown.
In Western blot, anti-FOXO4 antibody (ab128908) staining at 1/1000 dilution.
Exposure time: 59 seconds.
All lanes: Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] ab312326) at 1/1000 dilution
Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) starved overnight, then treated with 50 uM LY294092 for 30min whole cell lysate (untreated membrane) at 20 µg
Lane 2: Jurkat starved overnight, then treated with 20% serum and 100nM Calycin A for 30min whole cell lysate (untreated membrane) at 20 µg
Lane 3: Jurkat starved overnight, then treated with 20% serum and 100nM Calycin A for 30min whole cell lysate (phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75-80 kDa
Exposure time: 59s
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