Anti-FOXP1 antibody [EPR26484-79] KO Tested (ab314488)

Knockout Tested Rabbit Recombinant Monoclonal FOXP1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-FOXP1 antibody [EPR26484-79] (AB314488), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	Flow Cyt (Intra)	ICC/IF
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information FOXP1

Function

Transcriptional repressor (PubMed:18347093, PubMed:26647308). Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential (By similarity). Plays an important role in the specification and differentiation of lung epithelium. Acts cooperatively with FOXP4 to regulate lung secretory epithelial cell fate and regeneration by restricting the goblet cell lineage program; the function may involve regulation of AGR2. Essential transcriptional regulator of B-cell development. Involved in regulation of cardiac muscle cell proliferation. Involved in the columnar organization of spinal motor neurons. Promotes the formation of the lateral motor neuron column (LMC) and the preganglionic motor column (PGC) and is required for respective appropriate motor axon projections. The segment-appropriate generation of spinal cord motor columns requires cooperation with other Hox proteins. Can regulate PITX3 promoter activity; may promote midbrain identity in embryonic stem cell-derived dopamine neurons by regulating PITX3. Negatively regulates the differentiation of T follicular helper cells T(FH)s. Involved in maintenance of hair follicle stem cell quiescence; the function probably involves regulation of FGF18 (By similarity). Represses transcription of various pro-apoptotic genes and cooperates with NF-kappa B-signaling in promoting B-cell expansion by inhibition of caspase-dependent apoptosis (PubMed:25267198). Binds to CSF1R promoter elements and is involved in regulation of monocyte differentiation and macrophage functions; repression of CSF1R in monocytes seems to involve NCOR2 as corepressor (PubMed:15286807, PubMed:18347093, PubMed:18799727). Involved in endothelial cell proliferation, tube formation and migration indicative for a role in angiogenesis; the role in neovascularization seems to implicate suppression of SEMA5B (PubMed:24023716). Can negatively regulate androgen receptor signaling (PubMed:18640093). Acts as a transcriptional activator of the FBXL7 promoter; this activity is regulated by AURKA (PubMed:28218735). Isoform 8. Involved in transcriptional regulation in embryonic stem cells (ESCs). Stimulates expression of transcription factors that are required for pluripotency and decreases expression of differentiation-associated genes. Has distinct DNA-binding specifities as compared to the canonical form and preferentially binds DNA with the sequence 5'-CGATACAA-3' (or closely related sequences) (PubMed:21924763). Promotes ESC self-renewal and pluripotency (By similarity).

Alternative names

HSPC215, FOXP1, Forkhead box protein P1, Mac-1-regulated forkhead, MFH

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal FOXP1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26484-79
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOXP1 also known as forkhead box P1 is a transcription factor that belongs to the forkhead box (FOX) family. This protein weighs approximately 75-80 kDa and is expressed in a variety of tissues including the brain heart and lung. It plays a role in regulating gene expression acting as a transcriptional repressor or activator depending on the context. FOXP1 modulates several developmental processes through its interaction with DNA influencing cellular differentiation and proliferation. Its activity is vital in embryonic development and in maintaining the function of certain adult tissues.

Biological function summary

This transcription factor functions in the regulation of gene expression associated with neurodevelopment cardiac growth and immune responses. FOXP1 often forms a complex with other transcription factors such as FOXP2 and FOXP4 which enhances its regulatory roles. These interactions allow it to control various genetic programs that are important in the maturation and specification of different cell types. Moreover FOXP1 contributes to the modulation of B-cell development and function highlighting its importance in immune system regulation.

Pathways

FOXP1 takes part in several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. The Wnt signaling pathway relates FOXP1 to β-catenin influencing the expression of genes involved in cell proliferation and differentiation. In the TGF-beta pathway its interactions with SMAD proteins underline its function in cellular processes such as apoptosis and epithelial–mesenchymal transition. These pathways emphasize FOXP1's contribution to controlling cell growth and development across different biological contexts.

Associated diseases and disorders

Disruptions in the function of this transcription factor have been linked to conditions like intellectual disability and certain types of cancer including lymphoma. The relationship between FOXP1 and proteins such as BCL2 in lymphoma highlights its role in tumorigenesis and progression. Additionally mutations affecting FOXP1 can disturb its interaction with other forkhead family proteins leading to neurodevelopmental disorders that impact cognitive abilities and behavior. Understanding these connections provides insights into how FOXP1's altered activity can contribute to disease pathology.

Product promise

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Full details and terms and conditions can be found here:
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12 product images

Immunoprecipitation - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
FOXP1 was immunoprecipitated from 0.35 mg WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate with ab314488 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314488 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lane 2: ab314488 IP in WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314488 in WEHI-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/30 dilution
All lanes: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Immunoprecipitation - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
FOXP1 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab314488 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314488 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2: ab314488 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314488 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/30 dilution
All lanes: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver (PMID: 12692134, 11358962).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12692134, 11358962).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 70 kDa, 90 kDa
Exposure time: 26s
Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12692134, 11358962).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/1000 dilution
Lane 1: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 70 kDa, 90 kDa
Exposure time: 180s
Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: C2C12 (PMID: 12692134).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12692134, 11358962).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/1000 dilution
Lane 1: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 70 kDa, 90 kDa
Exposure time: 180s
Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: HDLM-2.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12692134, 11358962).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/1000 dilution
Lane 1: Ramos (human Burkitts lymphoma B lymphocyte) whole cell lysate at 40 µg
Lane 2: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 70 kDa, 90 kDa
Exposure time: 37s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling FOXP1 with ab314488 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat colon. The section was incubated with ab314488 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXP1 with ab314488 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse spleen. The section was incubated with ab314488 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling FOXP1 with ab314488 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse colon. The section was incubated with ab314488 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP1 with ab314488 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil. The section was incubated with ab314488 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXP1 with ab314488 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon (PMID: 32951006). The section was incubated with ab314488 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488)
Western blot: Anti-FOXP1 antibody [EPR26484-79] (ab314488) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab314488 was shown to bind specifically to FOXP1. A band was observed at 50-90 kDa in wild-type A549 cell lysates with no signal observed at this size in FOXP1 knockout cell line. To generate this image, wild-type and FOXP1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-FOXP1 antibody [EPR26484-79] (ab314488) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: FOXP1 knockout A549 cell lysate at 20 µg
Lane 3: HEK-293T cell lysate at 20 µg
Lane 4: SW480 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.