Anti-FOXP1 antibody [JC12]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (AB32010)

IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (AB32010)

IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Unknown

Western blot - Anti-FOXP1 antibody [JC12] (AB32010)

This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5) : 2816-24.

Image kindly provided by H. Hu.

All lanes:

Western blot - Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution

Lane 1:

BCBL1 human cell line

Lane 2:

Mutul human cell line

Predicted band size: 75 kDa

Observed band size: 60 kDa,65 kDa,80 kDa

false

• WB

Unknown

Western blot - Anti-FOXP1 antibody [JC12] (AB32010)

All lanes:

Western blot - Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/mL

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 Whole Cell Lysate Overexpressing FOXP1 at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

Predicted band size: 75 kDa

Observed band size: 100 kDa,80 kDa

false

• WB

CiteAb

Western blot - Anti-FOXP1 antibody [JC12] (AB32010)

FOXP1 western blot using anti-FOXP1 antibody [JC12] ab32010. Publication image and figure legend from Mendoza, E. & Scharff, C., 2017, Front Mol Neurosci, PubMed 28507505.

ab32010 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32010 please see the product overview.

Western blots demonstrating the specificity of the different FoxP antibodies by detecting only one FoxP protein in extracts of HeK293 cells transfected with an over-expression vector carrying either empty vector (E.V.), or the three FLAG-tagged FoxP proteins. Proteins were resolved by SDS-PAGE, transferred to nitrocellulose, and first detected (top panels) with anti-FoxP1 (A), or anti-FoxP2 (B), or anti FoxP4 (C) and sequentially detected FLAG/b-actin antibodies as loading controls (middle panels) (A–C). Bottom panels show detection of actin in the samples as a loading control. In all cases, the specificity of the antibody is evident from a single band in the expected lane (*).

false