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AB326729

Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free

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Rabbit Recombinant Monoclonal FOXP2 antibody. Carrier free. Suitable for ICC/IF, WB, IP and reacts with Mouse, Human samples.

View Alternative Names

Forkhead box protein P2, Foxp2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized SK-N-MC (human brain epithelial cell) cells labelling FOXP2 with ab326725 at 1/200 (2.495 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in SK-N-MC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab326725 at 1/200 dilution, followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling FOXP2 with ab326725 at 1/200 (2.495 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab326725 at 1/200 dilution, followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

Immunoprecipitation - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • IP

Lab

Immunoprecipitation - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725, the same antibody clone in a different buffer formulation.

FOXP2 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab326725 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326725 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MEF (mouse embryo fibroblast) whole cell lysate
Lane 2 : ab326725 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326725 in MEF whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-FOXP2 antibody [EPR29629-38] (<a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a>) at 1/1000 dilution

Lane 1:

MEF (mouse embryo fibroblast) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a> at 1/30 IP in MEF (mouse embryo fibroblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a> in MEF whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 80 kDa

false

Exposure time: 180s

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • WB

Lab

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane1 : 59 seconds, lane2 : 180 seconds

All lanes:

Western blot - Anti-FOXP2 antibody [EPR29629-38] (<a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a>) at 1/1000 dilution

Lane 1:

SK-N-MC (human brain epithelial cell) fresh whole cell lysate at 20 µg

Lane 2:

MEF (mouse embryo fibroblast) fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • WB

Lab

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FOXP2 antibody [EPR29629-38] (<a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control fresh whole cell lysate at 20 µg

Lane 2:

293T transfected with siRNA specifically targeting FOXP2 fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)
  • WB

Lab

Western blot - Anti-FOXP2 antibody [EPR29629-38] - BSA and Azide free (AB326729)

This data was developed using ab326725 ,the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) 1 : 10000 (124Kda).

All lanes:

Western blot - Anti-FOXP2 antibody [EPR29629-38] (<a href='/en-us/products/primary-antibodies/foxp2-antibody-epr29629-38-ab326725'>ab326725</a>) at 1/1000 dilution

Lane 1:

Mouse E12 embryo fresh tissue lysate at 60 µg

Lane 2:

Mouse E15 embryo fresh tissue lysate at 60 µg

Lane 3:

Mouse testis fresh tissue lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,124 kDa

false

Exposure time: 92s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29629-38

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "ChICCUTRUNseq-species-checked": "notRecommended", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>", "ChICCUTRUNseq-species-checked": "notRecommended", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "<p></p>" } } }
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Product details

ab326729 is the carrier-free version of ab326725.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Plays a role in synapse formation by regulating SRPX2 levels.
See full target information Foxp2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com