Mouse Recombinant Monoclonal FOXP3 antibody. Carrier free. Suitable for WB, mIHC, IHC-P and reacts with Human samples. Cited in 6 publications.
Images
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img99063.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048)")
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img47414.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img5053.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048)")
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img93000.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (AB96048)")
Publications
Filter by
- Neoplasma 70:747-7602023Tumor-derived autophagosome vaccines combined with immune adjuvants mediate antitumor immune responses via the neoantigen pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Yuan,Yue Chang,Yalan Dai,Yutong Chen,Rongbin Yue,Linjuan ZengPubMed 38014701
- iScience 26:1078912023Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMick J M van Eijs,José J M Ter Linde,Matthijs J D Baars,Mojtaba Amini,Miangela M Laclé,Eelco C Brand,Eveline M Delemarre,Julia Drylewicz,Stefan Nierkens,Rik J Verheijden,Bas Oldenburg,Yvonne Vercoulen,Karijn P M Suijkerbuijk,Femke van WijkPubMed 37766980
- Cold Spring Harbor molecular case studies 8:2022Tumor-immune microenvironment revealed by Imaging Mass Cytometry in a metastatic sarcomatoid urothelial carcinoma with a prolonged response to pembrolizumab.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHussein Alnajar,Hiranmayi Ravichandran,André Figueiredo Rendeiro,Kentaro Ohara,Wael Al Zoughbi,Jyothi Manohar,Noah Greco,Michael Sigouros,Jesse Fox,Emily Muth,Samuel Angiuoli,Bishoy Faltas,Michael Shusterman,Cora N Sternberg,Olivier Elemento,Juan Miguel MosqueraPubMed 35483877
- STAR protocols 3:1010342022MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniëlle Krijgsman,Neeraj Sinha,Matthijs J D Baars,Stephanie van Dam,Mojtaba Amini,Yvonne VercoulenPubMed 34977680
- Nature cancer 2:545-5622021Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F QuailPubMed 35122017
- Clinical cancer research : an official journal of the American Association for Cancer Research 25:1233-12382018Anti-CTLA-4 Immunotherapy Does Not Deplete FOXP3 Regulatory T Cells (Tregs) in Human Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnu Sharma,Sumit K Subudhi,Jorge Blando,Jorge Scutti,Luis Vence,Jennifer Wargo,James P Allison,Antoni Ribas,Padmanee SharmaPubMed 30054281
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | mIHC | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
Alternative names
Forkhead box protein P3, Scurfin, JM2, FOXP3, IPEX
Recommended products
Mouse Recombinant Monoclonal FOXP3 antibody. Carrier free. Suitable for WB, mIHC, IHC-P and reacts with Human samples. Cited in 6 publications.
Alternative names
Forkhead box protein P3, Scurfin, JM2, FOXP3, IPEX
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
236A/E7
- Purification technique
Affinity purification Protein A
- Specificity
The epitope recognized by FOXP3 antibody [236A/E7] (ab20034) is between amino acids 105-236. FOXP3 antibody [236A/E7] (ab20034) is expected to detect full length FOXP3 as well as both cleaved forms.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
This product has switched from a hybridoma to recombinant production method on 8th February 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
- Biological function summary
This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.
- Pathways
FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.
- Associated diseases and disorders
FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img99063.jpg "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048) at 1/1000 dilution
All lanes: Human mammary gland lysate at 20 µg
Predicted band size: 47 kDa
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img47414.jpg "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
Anti-FOXP3 antibody [236A/E7] ab20034 detects Human FOXP3 protein at ~50 kDa in HEK293T cells overexpressing the protein. It also detects FOXP3 in Human tonsil tissue lysate, however this band is significantly weaker in endogenous conditions. Upon higher exposure, weak bands can also be observed in HEK293T cell lysate.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-FOXP3 antibody [236A/E7] (Anti-FOXP3 antibody [236A/E7] ab20034; 5 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-mouse (green; 1:10000) for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048) at 5 µg/mL
Lane 1: HEK293T cell lysate at 20 µg
Lane 2: HEk293T cell lysate overexpressing Human FOXP3 at 20 µg
Lane 3: Human tonsil tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img5053.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
![Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--western-blot-img93000.jpg "Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048) at 1/1000 dilution
Lane 1: HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-Myc-tag, whole cell lysate at 10 µg
Lane 2: HEK-293 transfected with FOXP3 (WT) expression vector containing a GFP-Myc-tag, whole cell lysate at 10 µg
Predicted band size: 47 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401812.jpg "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
This data is courtesy of ImmunoAtlas and it can be found here.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436416.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
FOXP3 immunohistochemistry staining of human tonsil using mouse anti-FOXP3 antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-FOXP3 antibody [236A/E7] ab20034).
Immunohistochemical analysis of formalin fixed paraffin embedded) human tonsil labelling FOXP3 with Anti-FOXP3 antibody [236A/E7] ab20034 at a concentration of 2 µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. Anti-FOXP3 antibody [236A/E7] ab20034 anti-FOXP3 antibody [236A/E7] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401809.jpg "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
This data is courtesy of ImmunoAtlas and it can be found here.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436415.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
FOXP3 immunohistochemistry staining of human tonsil using mouse anti-FOXP3 antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-FOXP3 antibody [236A/E7] ab20034).
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling FOXP3 with Anti-FOXP3 antibody [236A/E7] ab20034 at a concentration of 2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). Anti-FOXP3 antibody [236A/E7] ab20034 anti-FOXP3 antibody [236A/E7] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401810.jpg "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
This data is courtesy of ImmunoAtlas and it can be found here.
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401811.jpg "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401808.jpg "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596; green; Opal™ 650) and Anti-FOXP3 (ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), Anti-CD8 alpha antibody [CAL66] - BSA and Azide free ab251596 (1/1500 dilution) and ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using Anti-FOXP3 antibody [236A/E7] ab20034, the same antibody clone in a different buffer formulation.
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401812.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401809.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436415.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401810.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401811.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
![Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-236a-e7-bsa-and-azide-free-ab96048--multiplex-immunohistochemistry-img401808.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)")
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