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AB36607

Anti-FOXP3 antibody [2A11G9]

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(6 Publications)

Mouse Monoclonal FOXP3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Transfected cell lysate - Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FOXP3.

View Alternative Names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (AB36607)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (AB36607)

ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.

Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (AB36607)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (AB36607)

Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-FOXP3 antibody [2A11G9] (AB36607)
  • WB

Unknown

Western blot - Anti-FOXP3 antibody [2A11G9] (AB36607)

All lanes:

Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution

Lane 1:

Protein expression of truncated FOXP3 in E. coli following transfection at 2 µg/mL

Lane 2:

Protein expression of truncated FOXP3 in HEK293 cells following transfection at 2 µg/mL

Predicted band size: 47 kDa

Observed band size: 50-53 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A11G9

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human FOXP3. The exact immunogen used to generate this antibody is proprietary information.

Q9BZS1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/200 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479, PubMed : 24835996, PubMed : 30513302, PubMed : 32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed : 23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed : 15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed : 17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed : 17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed : 18368049). Inhibits the transcriptional activator activity of RORA (PubMed : 18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
See full target information FOXP3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 23:111 PubMed40386306

2025

Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Ye,Xiujuan Zheng,Yali Yang,Ying Lyu

Scientific reports 9:15559 PubMed31664129

2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Yoon Park,Yongwoo Jang,Woori Kim,Joon Shin,Hui Ting Toh,Chun-Hyung Kim,Ho Sup Yoon,Pierre Leblanc,Kwang-Soo Kim

The Journal of biological chemistry 293:10287-10302 PubMed29739853

2018

PPE60 antigen drives Th1/Th17 responses via Toll-like receptor 2-dependent maturation of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Su,Zhen Zhang,Zijian Liu,Baozhou Peng,Cong Kong,Honghai Wang,Zhi Zhang,Ying Xu

Hypertension (Dallas, Tex. : 1979) 64:573-82 PubMed24935938

2014

Sex-specific T-cell regulation of angiotensin II-dependent hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Ji,Wei Zheng,Xiangjun Li,Jun Liu,Xie Wu,Monan Angela Zhang,Jason G Umans,Meredith Hay,Robert C Speth,Shannon E Dunn,Kathryn Sandberg

Molecular vision 19:1259-67 PubMed23805032

2013

Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes.

Applications

ICC/IF

Species

Mouse

Shahnawaz Imam,Raya B Elagin,Juan Carlos Jaume

Journal of immunology (Baltimore, Md. : 1950) 184:7257-67 PubMed20488788

2010

8-methoxypsoralen plus ultraviolet A therapy acts via inhibition of the IL-23/Th17 axis and induction of Foxp3+ regulatory T cells involving CTLA4 signaling in a psoriasis-like skin disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Tej Pratap Singh,Michael P Schön,Katrin Wallbrecht,Kai Michaelis,Beate Rinner,Gerlinde Mayer,Ulrike Schmidbauer,Heimo Strohmaier,Xiao-Jing Wang,Peter Wolf
View all publications
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