AB75763

Anti-FOXP3 antibody

Name: Anti-FOXP3 antibody (ab75763) | Abcam
Brand: Abcam Limited
SKU: AB75763
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(18 Publications)

Rabbit Polyclonal FOXP3 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Mouse Foxp3 aa 200-250.

View Alternative Names

Forkhead box protein P3, Scurfin, Foxp3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763)

Immunofluorescence of tumors from mice treated with MN-siPDL1 and gemcitabine. There was evidence of an increase in cell-mediated cytotoxicity, as evidenced by a decrease in the infiltration by immunosuppressive Foxp3 + regulatory T (Treg) cells detected using ab75763.

This image has been cropped from the origanal figure.

This image is taken from Fig 5. Byunghee Y. et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy, Sci Rep. 2019; 9: 4712. doi: 10.1038/s41598-019-41251-9. http://creativecommons.org/licenses/by/4.0/.

Supplier Data

Western blot - Anti-FOXP3 antibody (AB75763)

All lanes:

Western blot - Anti-FOXP3 antibody (ab75763) at 1/500 dilution

Lane 1:

rat kidney lysate

Lane 2:

rat cerebral cortex lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Foxp3 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q99JB6

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Deer": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed : 22813742). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed : 15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (By similarity). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed : 17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed : 18368049). Inhibits the transcriptional activator activity of RORA (By similarity). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (PubMed : 19696312).

See full target information Foxp3

style

left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 14:16 PubMed39955603

2025

Engineered oncolytic virus coated with anti-PD-1 and alendronate for ameliorating intratumoral T cell hypofunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yufu Zhu,Xuefeng Zhang,Jiaqi Jin,Xiaoqian Wang,Yang Liu,Jian Gao,Diancheng Hang,Lin Fang,Hengzhu Zhang,Hongmei Liu

Stem cell research & therapy 15:462 PubMed39627883

2024

Exosomes derived from minor salivary gland mesenchymal stem cells: a promising novel exosome exhibiting pro-angiogenic and wound healing effects similar to those of adipose-derived stem cell exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Xiang,Pengbing Ding,Jiaying Qian,Enhang Lu,Yimou Sun,Seyeon Lee,Zhenkun Zhao,Zhixuan Sun,Zhenmin Zhao

PloS one 19:e0307038 PubMed39150932

2024

Immunohistochemical analyses reveal FoxP3 expressions in spleen and colorectal cancer in mice treated with AOM/DSS, and their suppression by glycyrrhizin.

Applications

Unspecified application

Species

Unspecified reactive species

Guifeng Wang,Keiichi Hiramoto,Ning Ma,Shiho Ohnishi,Akihiro Morita,Yifei Xu,Nobuji Yoshikawa,Yasuo Chinzei,Mariko Murata,Shosuke Kawanishi

NPJ Regenerative medicine 9:7 PubMed38280914

2024

Matrix-bound nanovesicle-associated IL-33 supports functional recovery after skeletal muscle injury by initiating a pro-regenerative macrophage phenotypic transition.

Applications

Unspecified application

Species

Unspecified reactive species

J G Bartolacci,M N Behun,J P Warunek,T Li,A Sahu,G K Dwyer,A Lucas,J Rong,F Ambrosio,H R Turnquist,S F Badylak

Cancer immunology, immunotherapy : CII 72:2783-2797 PubMed37166485

2023

Exploiting docetaxel-induced tumor cell necrosis with tumor targeted delivery of IL-12.

Applications

Unspecified application

Species

Unspecified reactive species

S Elizabeth Franks,Ginette S Santiago-Sanchez,Kellsye P Fabian,Kristen Solocinski,Paul L Chariou,Duane H Hamilton,Joshua T Kowalczyk,Michelle R Padget,Sofia R Gameiro,Jeffrey Schlom,James W Hodge

Nature cardiovascular research 2:290-306 PubMed37621765

2023

Pairing of single-cell RNA analysis and T cell antigen receptor profiling indicates breakdown of T cell tolerance checkpoints in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Wang,Xi Zhang,Shu Lu,Chuankai Zhang,Zhe Ma,Rui Su,Yuanfang Li,Ting Sun,Yutao Li,Mingyang Hong,Xinyi Deng,Mohammad Rafiee Monjezi,Michael Hristov,Sabine Steffens,Donato Santovito,Klaus Dornmair,Klaus Ley,Christian Weber,Sarajo K Mohanta,Andreas J R Habenicht,Changjun Yin

International journal of molecular sciences 23: PubMed36362183

2022

CS12192, a Novel JAK3/JAK1/TBK1 Inhibitor, Synergistically Enhances the Anti-Inflammation Effect of Methotrexate in a Rat Model of Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyu Fang,Yiping Hu,Jiajing Dai,Lianhua He,Juan He,Bihua Xu,Xinle Han,Fubo Zhong,Huiyao Lan,Qingwen Wang

Journal of tissue engineering 13:20417314221118858 PubMed36003955

2022

Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Edina Sehic,Emy Thorén,Ingigerdur Gudmundsdottir,Mihai Oltean,Mats Brännström,Mats Hellström

Nature 605:152-159 PubMed35477759

2022

Neuroimmune cardiovascular interfaces control atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarajo K Mohanta,Li Peng,Yuanfang Li,Shu Lu,Ting Sun,Lorenzo Carnevale,Marialuisa Perrotta,Zhe Ma,Benjamin Förstera,Karen Stanic,Chuankai Zhang,Xi Zhang,Piotr Szczepaniak,Mariaelvy Bianchini,Borhan R Saeed,Raimondo Carnevale,Desheng Hu,Ryszard Nosalski,Fabio Pallante,Michael Beer,Donato Santovito,Ali Ertürk,Thomas C Mettenleiter,Barbara G Klupp,Remco T A Megens,Sabine Steffens,Jaroslav Pelisek,Hans-Henning Eckstein,Robert Kleemann,Livia Habenicht,Ziad Mallat,Jean-Baptiste Michel,Jürgen Bernhagen,Martin Dichgans,Giuseppe D'Agostino,Tomasz J Guzik,Peder S Olofsson,Changjun Yin,Christian Weber,Giuseppe Lembo,Daniela Carnevale,Andreas J R Habenicht

Frontiers in immunology 13:762580 PubMed35185872

2022

Indole-3-Acetic Acid Alters Intestinal Microbiota and Alleviates Ankylosing Spondylitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Shen,Lianjun Yang,Ke You,Tao Chen,Zhihai Su,Zhifei Cui,Min Wang,Weicong Zhang,Bin Liu,Kai Zhou,Hai Lu

View all publications

style

left-column

style

left-column

style

right-column