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Rabbit Polyclonal FOXP3 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Mouse Foxp3 aa 200-250.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763), expandable thumbnail
  • Western blot - Anti-FOXP3 antibody (AB75763), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (AB75763), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.02% Sodium azide

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Mouse Foxp3 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99JB6

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Predicted
Predicted
Mouse
Expected
Tested
Rat
Tested
Tested
Cat
Predicted
Predicted
Cow
Predicted
Predicted
Cynomolgus monkey
Predicted
Predicted
Deer
Predicted
Predicted
Monkey
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Cat, Human, Pig, Monkey, Cynomolgus monkey, Deer

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Predicted
Predicted

Species

Cow, Cat, Human, Pig, Monkey, Cynomolgus monkey, Deer

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:22813742). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (By similarity). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (By similarity). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (PubMed:19696312).

Alternative names

Recommended products

Rabbit Polyclonal FOXP3 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Mouse Foxp3 aa 200-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Mouse Foxp3 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99JB6
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763), expandable thumbnail
    This image is taken from Fig 5. Byunghee Y. et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy, Sci Rep. 2019; 9: 4712. doi: 10.1038/s41598-019-41251-9. http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763)

    Immunofluorescence of tumors from mice treated with MN-siPDL1 and gemcitabine. There was evidence of an increase in cell-mediated cytotoxicity, as evidenced by a decrease in the infiltration by immunosuppressive Foxp3 + regulatory T (Treg) cells detected using ab75763.

    This image has been cropped from the origanal figure.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

  • Western blot - Anti-FOXP3 antibody (ab75763), expandable thumbnail

    Western blot - Anti-FOXP3 antibody (ab75763)

    All lanes: Western blot - Anti-FOXP3 antibody (ab75763) at 1/500 dilution

    Lane 1: rat kidney lysate

    Lane 2: rat cerebral cortex lysate

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab75763)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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