Skip to main content

Rabbit Recombinant Monoclonal FOXP3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOXP3_HUMAN aa 250-350.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890), expandable thumbnail
  • Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890), expandable thumbnail
  • Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human FOXP3_HUMAN aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZS1

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 6μl/mg lysate.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal FOXP3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOXP3_HUMAN aa 250-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human FOXP3_HUMAN aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZS1
Clone number

BLR034F

Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

Associated diseases and disorders

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890), expandable thumbnail

    Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

  • Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890), expandable thumbnail

    Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    All lanes: Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution

    Lane 1: MJ whole cell lysate at 20 %

    Lane 2: Control IgG in MJ whole cell lysate

    Predicted band size: 47 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXP3 using ab243890 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890), expandable thumbnail

    Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    Detection: Chemiluminesence

    All lanes: Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg

    Lane 3: MJ whole cell lysate at 50 µg

    Lane 4: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

    Lane 5: RPMI-8226 whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 47 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com