Rabbit Recombinant Monoclonal FOXP3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOXP3_HUMAN aa 250-350.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
IHC-P | ICC | IP | WB | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use 6μl/mg lysate. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
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Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
IPEX, JM2, JM2, FOXP3, IPEX, Forkhead box protein P3, Scurfin
Rabbit Recombinant Monoclonal FOXP3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOXP3_HUMAN aa 250-350.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
BLR034F
Recombinant antibody was purified from cell culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is sold under License from Bethyl Laboratories, Inc.
This supplementary information is collated from multiple sources and compiled automatically.
FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.
FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.
FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
All lanes: Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1: MJ whole cell lysate at 20 %
Lane 2: Control IgG in MJ whole cell lysate
Predicted band size: 47 kDa
Exposure time: 30s
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXP3 using ab243890 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Detection: Chemiluminesence
All lanes: Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg
Lane 3: MJ whole cell lysate at 50 µg
Lane 4: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
Lane 5: RPMI-8226 whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 47 kDa
Exposure time: 30s
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