Rabbit Recombinant Monoclonal FOXP3 antibody. Carrier free. Suitable for mIHC, IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Images
![Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunoprecipitation-img3484.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)")
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img68254.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)")
![Western blot - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--western-blot-img5249.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76735.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)")
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img67227.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IHC-P | IP | WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Tested | Expected | Tested | Not recommended | Not recommended | Tested |
| Rat | Expected | Tested | Expected | Expected | Not recommended | Not recommended | Tested |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Please adjust the dilution factor according to tissues from different species. Recommend performing IHC staining on a Leica Biosystems BOND® RX instrument because BOND® RX instrument assay is stronger than overnight incubation assay. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Please adjust the dilution factor according to tissues from different species. Recommend performing IHC staining on a Leica Biosystems BOND® RX instrument because BOND® RX instrument assay is stronger than overnight incubation assay. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Please adjust the dilution factor according to tissues from different species. Recommend performing IHC staining on a Leica Biosystems BOND® RX instrument because BOND® RX instrument assay is stronger than overnight incubation assay. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Anti-FOXP3 antibody [EPR22102-37] ab215206 is not suitable for endogenous FOXP3 detection in tissues. |
Species Mouse | Dilution info - | Notes According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don't have experimental data to support that. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
Alternative names
Forkhead box protein P3, Scurfin, JM2, FOXP3, IPEX
Recommended products
Rabbit Recombinant Monoclonal FOXP3 antibody. Carrier free. Suitable for mIHC, IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22102-37
- Purification technique
Affinity purification Protein A
- Specificity
The rat recommendation is based on the IHC results. We do not guarantee WB for rat.
According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don't have experimental data to support that.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab244242 is the carrier-free version of Anti-FOXP3 antibody [EPR22102-37] ab215206.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
- Biological function summary
This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.
- Pathways
FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.
- Associated diseases and disorders
FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunoprecipitation-img3484.jpg "Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
FOXP3 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate 10 μg (Input).
Lane 2: Anti-FOXP3 antibody [EPR22102-37] ab215206 IP in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FOXP3 antibody [EPR22102-37] ab215206 in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.FOXP3 isoforms have been described in the literature. (PMID: 19568423).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
All lanes: Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] (Anti-FOXP3 antibody [EPR22102-37] ab215206)
Predicted band size: 47 kDa, 90 kDa
Observed band size: 55-73 kDa
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img68254.jpg "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with a GFP-tagged FOXP3 expression construct labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/40 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor®647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Western blot - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--western-blot-img5249.jpg "Western blot - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Western blot - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Blocking buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
Lane 1: Western blot - Anti-FOXP3 antibody [EPR22102-37] (Anti-FOXP3 antibody [EPR22102-37] ab215206) at 1/200 dilution
Lane 2: Western blot - Anti-FOXP3 antibody [EPR22102-37] (Anti-FOXP3 antibody [EPR22102-37] ab215206) at 1/1000 dilution
All lanes: Full-length C-MYC/DDK tagged Recombinant Mouse Foxp3 protein 10ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
Exposure time: 180s
Blocking buffer and concentration: 5% NFDM /TBST
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76735.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on regulatory T-cells in human thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img67227.jpg "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Human PBMCs were stained with Alexa Fluor® 647 conjugated anti-human CD4 and BV421 conjugated anti-human CD25. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab98462, Dylight®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.
Gating strategy and expression pattern is consistent with literature (PMID: 27330808).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img12480.jpg "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Mouse splenocytes were stained with Alexa Fluor®647 conjugated anti-mouse CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilisation buffer, followed by intracellular staining with rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077,Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img18624.jpg "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Rat splenocytes were stained with Alexa Fluor®647 conjugated anti-rat CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34389.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on regulatory T-cells in rat thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126322.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on regulatory T-cells in mouse spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28292.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on regulatory T-cells in mouse thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106677.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on regulatory T-cells in human spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img406477.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling FOXP3 with Anti-FOXP3 antibody [EPR22102-37] ab215206 at 1:100 dilution (3.67 μg/ml).
Image A: LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with Anti-FOXP3 antibody [EPR22102-37] ab215206 for 30min at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.
Image B: Goat Anti-Rabbit IgG H&L (HRP polymer) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with Anti-FOXP3 antibody [EPR22102-37] ab215206 overnight at +4 °C. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FOXP3 antibody [EPR22102-37] ab215206).
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img12480.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--flow-cytometry-intracellular-img18624.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34389.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126322.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28292.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106677.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242), expandable thumbnail](https://content.abcam.com/products/images/foxp3-antibody-epr22102-37-bsa-and-azide-free-ab244242--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img406477.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)")
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