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Mouse Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 23 publications.


Images

Western blot - Anti-FOXP3 antibody [mAbcam 450] (AB450), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (AB450), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (AB450), expandable thumbnail

Publications

Key facts

Isotype

IgG3

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer.It did not show positive staining with cryostat or paraffin-embedded human tonsil.

Tested
Tested

Species

Human

Dilution info

5 µg/cells

Notes

-

Associated Products

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Target data

Function

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

Alternative names

Recommended products

Mouse Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 23 publications.

Key facts

Isotype

IgG3

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

mAbcam 450

Purification technique

Affinity purification Protein A

Specificity

Detects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450), expandable thumbnail

    Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)

    All lanes: Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450) at 1 µg/mL

    All lanes: HEK293 cells overexpressing human FOXP3 at 20 µg

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 50 kDa

    Exposure time: 45s

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

    IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab450, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

    IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab450, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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