Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human cardiac muscle.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human heart (fresh) tissue labeling FOXP3 with ab309108 at 1/10000 dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on human heart. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309108 overnight at 4°C. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)at 1/1000 2 ug/mL dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymp tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Scattered nuclear staining on human Hodgkin's lymphoma.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Scattered nuclear staining on human tonsil.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human tonsil (fresh) tissue labeling FOXP3 with ab309108 at 1/10000 dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on human tonsil. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309108 overnight at 4°C. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)at 1/1000 2 ug/mL dilution.

• WB

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Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed was in consistant with the literature (PMID : 17586580). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108) at 1/1000 dilution

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Observed band size: 72 kDa

false

Exposure time: 180s