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Mouse Recombinant Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Transfected cell lysate - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108), expandable thumbnail
  • Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (AB309108), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Expected
Tested
Tested
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the FOXP3 gene is a transcriptional regulator critical for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in immune system homeostasis by ensuring the suppressive function and stability of Treg cells and directly influencing conventional T-cells' expansion and function. FOXP3 can function as either a transcriptional repressor or activator, depending on its interactions with other factors, such as transcription factors, histone acetylases, and deacetylases. It co-activates genes like CTLA4 and TNFRSF18 and represses cytokine genes, including interleukin-2 (IL2) and interferon-gamma (IFNG), and inhibits cytokine production by repressing the activity of transcription factors RELA and NFATC2. FOXP3's association with histone acetylase KAT5 and deacetylase HDAC7 mediates IL2 repression, while its interaction with transcription factor RUNX1 modulates the expression of TNFRSF18, IL2RA, CTLA4, IL2, and IFNG. It also antagonizes RORC to inhibit the differentiation of IL17 producing helper T-cells (Th17), leading to reduced IL17 expression and promoting Treg development, while inhibiting the transcriptional activator activity of RORA. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

Recommended products

Mouse Recombinant Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbcam 450
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

Associated diseases and disorders

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cardiac muscle using mouse Anti-FOXP3 antibody

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human cardiac muscle.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Hodgkin's lymphoma using mouse Anti-FOXP3 antibody

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymp tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Scattered nuclear staining on human Hodgkin's lymphoma.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil using mouse Anti-FOXP3 antibody

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab309108 at 1/500 (2.132 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Scattered nuclear staining on human tonsil.The section was incubated with ab309108 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Western blot staining using mouse Anti-FOXP3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The molecular weight observed was in consistant with the literature (PMID: 17586580).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108) at 1/1000 dilution

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

    Observed band size: 72 kDa

    Exposure time: 180s

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Immunohistochemistry (Frozen sections) staining of Human heart (fresh) using mouse Anti-FOXP3 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human heart (fresh) tissue labeling FOXP3 with ab309108 at 1/10000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on human heart. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309108 overnight at 4°C. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG2a (ab309108)

    FOXP3 Immunohistochemistry (Frozen sections) staining of Human tonsil (fresh) using mouse Anti-FOXP3 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human tonsil (fresh) tissue labeling FOXP3 with ab309108 at 1/10000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on human tonsil. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309108 overnight at 4°C. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)at 1/1000 2 ug/mL dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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