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Mouse Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 23 publications.


Images

Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (AB450), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (AB450), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (AB450), expandable thumbnail

Publications

Key facts

Isotype
IgG3
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer.It did not show positive staining with cryostat or paraffin-embedded human tonsil.

Tested
Tested

Species
Human
Dilution info
5 µg/cells
Notes

-

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Target data

Function

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

Alternative names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

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Mouse Monoclonal FOXP3 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 23 publications.

Key facts

Isotype
IgG3
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbcam 450
Purification technique
Affinity purification Protein A
Specificity

Detects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

Associated diseases and disorders

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

Product promise

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3 product images

  • Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450), expandable thumbnail

    Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450)

    FOXP3 Western blot staining of HEK293 cells overexpressing human FOXP3 using mouse Anti-FOXP3 antibody

    All lanes: Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450) at 1 µg/mL

    Lane 1: HEK293 cells overexpressing human FOXP3 at 20 µg

    Lane 2: HEK293 cells overexpressing human FOXP3 at 20 µg with Human FOXP3 peptide (ab16809)

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 50 kDa

    Exposure time: 45s

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450)

    FOXP3 Immunohistochemistry (Frozen sections) staining using mouse Anti-FOXP3 antibody

    IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab450, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (ab450)

    FOXP3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-FOXP3 antibody

    IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab450, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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