Anti-FOXP4 antibody [EPR22714-2] (ab242127)

Rabbit Recombinant Monoclonal FOXP4 antibody. Suitable for IHC-P, ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-FOXP4 antibody [EPR22714-2] (AB242127), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Expected	Tested
Mouse	Tested	Expected	Expected	Tested	Tested	Expected
Rat	Tested	Expected	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information FOXP4

Function

Transcriptional repressor that represses lung-specific expression.

Alternative names

FKHLA, FOXP4, Forkhead box protein P4, Fork head-related protein-like A

Recommended products

Rabbit Recombinant Monoclonal FOXP4 antibody. Suitable for IHC-P, ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22714-2
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FOXP4 also known as Forkhead Box P4 is a transcription factor belonging to the FOX protein family. Its molecular mass approximately 80 kDa. It is expressed in various tissues with high expression levels observed in lung kidney and prostate. The protein binds to DNA and regulates the transcription of genes involved in development and differentiation.

Biological function summary

FOXP4 plays an important role in regulating developmental processes particularly in the central nervous system and lung tissue. It often functions as part of a complex that includes other FOXP proteins like FOXP1 and FOXP2 which share a conserved DNA-binding forkhead domain. These proteins collaborate to regulate genes important for cell differentiation and organogenesis.

Pathways

The transcriptional activity of FOXP4 integrates into significant signaling pathways such as the Wnt signaling and TGF-beta pathways. These pathways govern processes like cellular growth and differentiation. FOXP4 interacts with other FOX proteins including FOXP1 to modulate gene expression patterns important for these pathways.

Associated diseases and disorders

Research indicates that FOXP4 is associated with lung cancer and neurodevelopmental disorders. Its dysregulation can affect cellular processes linked to tumorigenesis and cognitive development. FOXP4’s involvement with other proteins such as FOXP1 highlights its role in modulating gene networks that when disrupted can contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The lysates were kindly provided by our collaborator Dr. Bin Zhao, Zhejiang University.
Lanes 1-2: Merged signal (red and green). Green - ab242127 observed at 80 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab242127 Anti-FOXP4 antibody [EPR22714-2] was shown to react with FOXP4 in HepG2 cells in Western blot. Loss of signal was observed when FOXP4 knockout sample was used. Wild-type and FOXP4 knockout samples were subjected to SDS-PAGE. ab242127 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2: FOXP4 knock out HepG2 whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa
Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: Lanes 1-2: 59 seconds; Lanes 3-4: 3 minutes.
All lanes: Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa
Immunoprecipitation - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
FOXP4 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab242127 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242127 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab242127 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab242127 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Predicted band size: 73 kDa
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human tonsil. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument, Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labelling FOXP4 with ab242127 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HepG2 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Bands around 50 kDa and 37 kDa in lane 3 are caused by degradation.
Exposure time: 3 minutes
All lanes: Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human tonsil tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
Secondary
Lanes 1 - 2: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lane 3: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse spleen. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling FOXP4 with ab242127 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 3 minutes
All lanes: Western blot - Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat spleen. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-FOXP4 antibody [EPR22714-2] (ab242127)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab242127 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.